Evidence map›Paper›PMID 30128603›Full record

ArticlePlanta2018

Sanger and next generation sequencing in the characterisation of arbuscular mycorrhizal fungi (AMF) in Pancratium maritimum L. (Amaryllidaceae), a representative plant species of Mediterranean sand dunes.

Olga De Castro, Mariano Avino, Antonietta Di Maio, Bruno Menale, Marco Guida

Abstract read
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In one paragraph

Article in Planta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Olga De CastroDepartment of Biology, University of Naples Federico II, Via Foria 223, Botanical Garden, 80139, Naples, Italy. olga.decastro@unina.it.ORCID http://orcid.org/0000-0002-4861-3960
Mariano AvinoDepartment of Pathology and Laboratory Medicine, Western University, Dental Sciences Building, London, N6A 5C1, Canada.
Antonietta Di MaioDNATech srl, Spin-off Company of the University of Naples Federico II, Botanical Garden, Via Foria 223, 80139, Naples, Italy.
Bruno MenaleDepartment of Biology, University of Naples Federico II, Via Foria 223, Botanical Garden, 80139, Naples, Italy.
Marco GuidaDNATech srl, Spin-off Company of the University of Naples Federico II, Botanical Garden, Via Foria 223, 80139, Naples, Italy.

Funding

Nando and Elsa Peretti Foundation 2012-83
6 · The paper itself

Abstract

MAIN

conclusionAn interesting AMF colonization microcosm has been detected in the roots of Pancratium maritimum (sea daffodil). Both sequencing techniques (Sanger and NGS) have been used for AMF characterisation, showing a balanced trade-off between pros and cons. By Sanger and next generation sequencing of rRNA nuclear molecular markers (SSU-ITS-LSU and ITS2, respectively), the presence of AMF communities in the roots of P. maritimum was evaluated. Our results shed light on the presence of AMF in sea daffodil and the diversity of assemblages of AMF detected after Sanger sequencing of the SSU-ITS-LSU marker is much higher than that determined following NGS sequencing of ITS2 alone.

Indexed as

AmaryllidaceaeDNA Barcoding, TaxonomicDNA, FungalDNA, RibosomalFungiHigh-Throughput Nucleotide SequencingMycorrhizaePlant RootsSequence Analysis, DNADNA, FungalDNA, RibosomalDNA barcodingIllumina MiSeqITS2Psammophilic plantRootSea daffodilSSU–ITS–LSU

Identifiers

PMID30128603

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.