ArticleScience advances2018
Sequence-programmable covalent bonding of designed DNA assemblies.
Article in Science advances, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 119 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
119 citing papers in PubMed, 270 citations in OpenAlex.
- Chemical modifications to enhance the stability of DNA nanoparticles for biological applications.iScience · 2026Review
- Programmable Functional Silicification of DNA Origami Nanostructures.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- DNA origami-based drug delivery and cell manipulation: toward intelligent nanomedicine.RSC chemical biology · 2026Review
- Recent Advances in Conjugation Chemistry Expanding the Applications of the DNA Tetrahedron.Chemical biology & drug design · 2026Review
- From Blueprint to Breakthrough: How Far Can We Fold DNA Origami for Nano-Enabled Technologies?JACS Au · 2026Review
- Tunable DNA Origami Nanosensors for Detection of Multiscale Spatial Ion Concentration Gradients.ACS sensors · 2026Article
- Stable and programmable formation of DNA nanostructures via inter-strand crosslinking by assembly-triggered oxanine and amine linkage.Nucleic acids research · 2026Article
- Membrane-Spanning Nanopores Formed from Nucleic Acids.Chemical reviews · 2026Review
- Tools For Building Artificial Biological Nanostructures.ACS nano · 2026Review
- Light-responsive DNA nanostructures.Nature reviews. Chemistry · 2026Review
- Vesicle-Templated Self-Assembly of Programmable Freestanding Multi-μm DNA Shells.Nano letters · 2026Article
- Dephosphorylation-regulated MNAzyme-PER cascade as a programmable converter for multi-task molecular actuation.Journal of nanobiotechnology · 2026Article
- Tetrahedral framework nucleic acids as a promising vehicle for small molecule drugs of traditional Chinese medicine: application to disease prevention and treatment.Journal of nanobiotechnology · 2026Review
- KIMMDY: a biomolecular reaction emulator.Nature communications · 2026Article
- DNA Nanostructure-Templated Multivalency Enables Broad-Spectrum Virus Inhibition.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Self-assembled cell-scale containers made from DNA origami membranes.Nature materials · 2026Article
- Uncovering Design and Assembly Rules for mRNA-DNA Origami.Nano letters · 2026Article
- Resolving DNA origami structural integrity and pharmacokinetics in vivo.Nature nanotechnology · 2026Article
- DNA Origami-Templated Aptamer Chiral Structures Realize Cellular Enantioselectivity.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- DNA Origami and Its Applications in Synthetic Biology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
59 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bottom-up fabrication of custom nanostructures using the methods of DNA nanotechnology has great potential for applications in many areas of science and technology. One obstacle to applications concerns the constrained environmental conditions at which DNA objects retain their structure. We present a general, site-selective, and scalable method for creating additional covalent bonds that increase the structural stability of DNA nanostructures. Placement of thymidines in close proximity within DNA nanostructures allows the rational creation of sites for covalent cyclobutane pyrimidine dimer (CPD) bonds induced via ultraviolet irradiation. The additional covalent bonds may be used in a sequence-programmable fashion to link free strand termini, to bridge strand breaks at crossover sites, and to create additional interhelical connections. Thus designed multilayer DNA origami objects can remain stable at temperatures up to 90°C and in pure double-distilled water with no additional cations present. In addition, these objects show enhanced resistance against nuclease activity. Cryo-electron microscopy (cryo-EM) structural analysis of non-cross-linked and cross-linked objects indicated that the global shape and the internal network of crossovers are preserved after irradiation. A cryo-EM map of a CPD-stabilized multilayer DNA origami object determined at physiological ionic strength reveals a substantial swelling behavior, presumably caused by repulsive electrostatic forces that, without covalent stabilization, would cause disassembly at low ionic strength. Our method opens new avenues for applications of DNA nanostructures in a wider range of conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.