ArticleTranslational neurodegeneration2018
Ultra-High Field Diffusion MRI Reveals Early Axonal Pathology in Spinal Cord of ALS mice.
Article in Translational neurodegeneration, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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16 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Diffusion Tensor Imaging-Based Studies at the Group-Level Applied to Animal Models of Neurodegenerative Diseases.Frontiers in neuroscience · 2020Pooled it
- Encephalography cross-frequency coupling and brain alteration in amyotrophic lateral sclerosis.Brain communications · 2025Article
- Tract-specific analysis of diffusion MRI at 3T detects cervical spinal cord aberrations in multiple sclerosis.Imaging neuroscience (Cambridge, Mass.) · 2025Article
- Spinal cord morphology and PKD2L1Frontiers in neuroanatomy · 2025Article
- Non-coding RNAs and Aquaporin 4: Their Role in the Pathogenesis of Neurological Disorders.Neurochemical research · 2024Review
- Integrating multimodality magnetic resonance imaging to the Allen Mouse Brain Common Coordinate Framework.NMR in biomedicine · 2023Article
- Variation in Diffusion Tensor Imaging Parameters in the Cervical and Thoracic Spinal Cord (C1-C5 and C6-T2) Segments of Normal Beagle Dogs.Veterinary sciences · 2023Article
- Editorial: Translatable Models and MRI Methods for Neurodegenerative Diseases.Frontiers in neuroscience · 2022Article
- Overexpression of miR-124 in Motor Neurons Plays a Key Role in ALS Pathological Processes.International journal of molecular sciences · 2021Article
- In vivo detection of microstructural spinal cord lesions in dogs with degenerative myelopathy using diffusion tensor imaging.Journal of veterinary internal medicine · 2021Article
- Editorial: Innovative Imaging Techniques in Preclinical Models of Neurodegenerative Diseases.Frontiers in neuroscience · 2021Article
- Assessing neuraxial microstructural changes in a transgenic mouse model of early stage Amyotrophic Lateral Sclerosis by ultra-high field MRI and diffusion tensor metrics.Animal models and experimental medicine · 2020Article
- Article
- Generation of Mouse Basal Ganglia Diffusion Tractography Using 9.4T MRI.Experimental neurobiology · 2019Article
- Brain-Immune Interactions and Neuroinflammation After Traumatic Brain Injury.Frontiers in neuroscience · 2019Review
- Neurite orientation dispersion and density imaging can detect presymptomatic axonal degeneration in the spinal cord of ALS mice.Functional neurologyArticle
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAmyotrophic lateral sclerosis (ALS) is a disease characterized by a progressive degeneration of motor neurons leading to paralysis. Our previous MRI diffusion tensor imaging studies detected early white matter changes in the spinal cords of mice carrying the G93A-SOD1 mutation. Here, we extend those studies using ultra-high field MRI (17.6 T) and fluorescent microscopy to investigate the appearance of early structural and connectivity changes in the spinal cords of ALS mice.
methodsThe spinal cords from presymptomatic and symptomatic mice (80 to 120 days of age) were scanned (ex-vivo) using diffusion-weighted MRI. The fractional anisotropy (FA), axial (AD) and radial (RD) diffusivities were calculated for axial slices from the thoracic, cervical and lumbar regions of the spinal cords. The diffusion parameters were compared with fluorescence microscopy and membrane cellular markers from the same tissue regions.
resultsAt early stages of the disease (day 80) in the lumbar region, we found, a 19% decrease in FA, a 9% decrease in AD and a 35% increase in RD. Similar changes were observed in cervical and thoracic spinal cord regions. Differences between control and ALS mice groups at the symptomatic stages (day 120) were larger. Quantitative fluorescence microscopy at 80 days, demonstrated a 22% reduction in axonal area and a 22% increase in axonal density. Tractography and quantitative connectome analyses measured by edge weights showed a 52% decrease in the lumbar regions of the spinal cords of this ALS mice group. A significant increase in ADC (23.3%) in the ALS mice group was related to an increase in aquaporin markers.
conclusionsThese findings suggest that the combination of ultra-high field diffusion MRI with fluorescent ALS mice reporters is a useful approach to detect and characterize presymptomatic white matter micro-ultrastructural changes and axonal connectivity anomalies in ALS.
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