ArticleJournal of biological engineering2018
A novel method for segmenting growth of cells in sheared endothelial culture reveals the secretion of an anti-inflammatory mediator.
Article in Journal of biological engineering, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The trial behind it
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Human Cell-Derived Extracellular Matrix Modulates Endothelial Cell Morphology and Metabolism in Response to Fluid Shear Stress.ACS biomaterials science & engineering · 2026Article
- Computational Optimization of the Orbital Shaker Operational Parameters in Endothelial Mechanobiology.Annals of biomedical engineering · 2026Article
- Endothelial dysfunction in cardiovascular diseases: mechanisms and in vitro models.Molecular and cellular biochemistry · 2025Review
- 12/15-lipoxygenase mediates disturbed flow-induced endothelial dysfunction and atherosclerosis.Molecular medicine (Cambridge, Mass.) · 2025Article
- Basal endothelial glycocalyx's response to shear stress: a review of structure, function, and clinical implications.Frontiers in cell and developmental biology · 2024Review
- Mechanobiology of the endothelium in vascular health and disease: in vitro shear stress models.Cardiovascular drugs and therapy · 2023Review
- Insight into muscle stem cell regeneration and mechanobiology.Stem cell research & therapy · 2023Review
- Modification of the swirling well cell culture model to alter shear stress metrics.Biotechnology and bioengineering · 2023Article
- Disturbed flow increases endothelial inflammation and permeability via a Frizzled-4-β-catenin-dependent pathway.Journal of cell science · 2023Article
- Single-Cell RNA sequencing investigation of female-male differences under PAD conditions.Frontiers in cardiovascular medicine · 2023Article
- Characterizing nuclear morphology and expression of eNOS in vascular endothelial cells subjected to a continuous range of wall shear stress magnitudes and directionality.Journal of the mechanical behavior of biomedical materials · 2023Article
- Influence of right coronary artery motion, flow pulsatility and non-Newtonian rheology on wall shear stress metrics.Frontiers in bioengineering and biotechnology · 2022Article
- Engineering solutions for biological studies of flow-exposed endothelial cells on orbital shakers.PloS one · 2022Article
- Haemodynamic Wall Shear Stress, Endothelial Permeability and Atherosclerosis-A Triad of Controversy.Frontiers in bioengineering and biotechnology · 2022Review
- S1P in the development of atherosclerosis: roles of hemodynamic wall shear stress and endothelial permeability.Tissue barriers · 2021Review
- Interferons Are Pro-Inflammatory Cytokines in Sheared-Stressed Human Aortic Valve Endothelial Cells.International journal of molecular sciences · 2021Article
- Endothelial cells exposed to atheroprotective flow secrete follistatin-like 1 protein which reduces transcytosis and inflammation.Atherosclerosis · 2021Article
- Leucine-Rich α-2-Glycoprotein 1 Suppresses Endothelial Cell Activation Through ADAM10-Mediated Shedding of TNF-α Receptor.Frontiers in cell and developmental biology · 2021Article
- Notch in mechanotransduction - from molecular mechanosensitivity to tissue mechanostasis.Journal of cell science · 2020Review
- Computational Characterization of The Dish-In-A-Dish, A High Yield Culture Platform for Endothelial Shear Stress Studies on the Orbital Shaker.Micromachines · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
backgroundEffects of shear stress on endothelium are important for the normal physiology of blood vessels and are implicated in the pathogenesis of atherosclerosis. They have been extensively studied in vitro. In one paradigm, endothelial cells are cultured in devices that produce spatially varying shear stress profiles, and the local profile is compared with the properties of cells at the same position. A flaw in this class of experiments is that cells exposed to a certain shear profile in one location may release mediators into the medium that alter the behaviour of cells at another location, experiencing different shear, thus obscuring or corrupting the true relation between shear and cell properties.
methodsSurface coating methods were developed for attaching cells only to some areas of culture-ware and preventing them from spreading into other regions even during prolonged culture.
resultsSegmenting the growth of cells had no effect on cell shape, alignment and number per unit area compared to culturing cells in the whole well, but there were differences in tumour-necrosis-factor-α (TNF-α)-induced expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1), and monocyte adherence to the monolayer.
conclusionsThe results are consistent with the release of a mediator from cells exposed to high-magnitude uniaxial shear stress that has anti-inflammatory effects on activated endothelium; the mediator may be of importance in atherogenesis. Hence the new methods revealed an important property that would not have been observed without growth segmentation, suggesting that they could find more widespread application.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.