Evidence map›Paper›PMID 30127420›Full record

ArticleNature communications2018

Monolayer surface chemistry enables 2-colour single molecule localisation microscopy of adhesive ligands and adhesion proteins.

Xun Lu, Philip R Nicovich, Manchen Zhao, Daniel J Nieves, Mahdie Mollazade, S R C Vivekchand, Katharina Gaus, J Justin Gooding

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Electrochemical Dye Switching Assisted Spectral Demixing and 3D STORM Imaging.Angewandte Chemie (International ed. in English) · 2026
    Article
  2. Gelatin Methacrylate Macroporous Cell Scaffold Fabrication via One-Pot Aqueous Two-Phase Separation.Journal of biomedical materials research. Part B, Applied biomaterials · 2025
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xun LuSchool of Chemistry, Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of New South Wales, Sydney, NSW, 2052, Australia.
Philip R NicovichEMBL Australia Node in Single Molecule Science, School of Medical Sciences and the ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, NSW, 2052, Australia.
Manchen ZhaoSchool of Chemistry, Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of New South Wales, Sydney, NSW, 2052, Australia.
Daniel J NievesEMBL Australia Node in Single Molecule Science, School of Medical Sciences and the ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, NSW, 2052, Australia.
Mahdie MollazadeEMBL Australia Node in Single Molecule Science, School of Medical Sciences and the ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, NSW, 2052, Australia.
S R C VivekchandSchool of Chemistry, Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of New South Wales, Sydney, NSW, 2052, Australia.
Katharina GausEMBL Australia Node in Single Molecule Science, School of Medical Sciences and the ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, NSW, 2052, Australia. k.gaus@unsw.edu.au.
J Justin GoodingSchool of Chemistry, Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of New South Wales, Sydney, NSW, 2052, Australia. justin.gooding@unsw.edu.au.

Funding

Australian Research Council (ARC) CE140100036Department of Health | National Health and Medical Research Council (NHMRC) 1037320Department of Health | National Health and Medical Research Council (NHMRC) 1091261
6 · The paper itself

Abstract

Nanofabricated and nanopatterned surfaces have revealed the sensitivity of cell adhesion to nanoscale variations in the spacing of adhesive ligands such as the tripeptide arginine-glycine-aspartic acid (RGD). To date, surface characterisation and cell adhesion are often examined in two separate experiments so that the localisation of ligands and adhesion proteins cannot be combined in the same image. Here we developed self-assembled monolayer chemistry for indium tin oxide (ITO) surfaces for single molecule localisation microscopy (SMLM). Cell adhesion and spreading were sensitive to average RGD spacing. At low average RGD spacing, a threshold exists of 0.8 RGD peptides per µm

Indexed as

AdhesivesAnimalsCell AdhesionCell Adhesion MoleculesColorGlassLigandsMiceMicroscopyNIH 3T3 CellsOligopeptidesPaxillinSingle Molecule ImagingSurface PropertiesTin CompoundsAdhesivesarginyl-glycyl-aspartic acidCell Adhesion Moleculesindium tin oxideLigandsOligopeptidesPaxillinTin Compounds

Identifiers

PMID30127420
PMCPMC6102261

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.