ArticleScientific reports2018
Image-Assisted Microvessel-on-a-Chip Platform for Studying Cancer Cell Transendothelial Migration Dynamics.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Advanced Microfluidic Models of Cancer and Immune Cell Extravasation: A Systematic Review of the Literature.Frontiers in bioengineering and biotechnology · 2020Pooled it
- Microfluidic 3D cell culture: potential application of collagen hydrogels with an optimal dose of bioactive glasses.Scientific reports · 2025Article
- Establishment and evaluation of on-chip intestinal barrier biosystems based on microfluidic techniques.Materials today. Bio · 2024Review
- Biofabrication methods for reconstructing extracellular matrix mimetics.Bioactive materials · 2024Review
- The impact of pericytes on the stability of microvascular networks in response to nanoparticles.Scientific reports · 2023Article
- Review
- In Vitro Flow Chamber Design for the Study of Endothelial Cell (Patho)Physiology.Journal of biomechanical engineering · 2022Review
- Microfluidic vascular models of tumor cell extravasation.Frontiers in oncology · 2022Review
- On-chip perivascularLab on a chip · 2021Article
- Biofabrication of tissue engineering vascular systems.APL bioengineering · 2021Review
- From Protein Building Blocks to Functional Materials.ACS nano · 2021Review
- The Cancer Microenvironment: Mechanical Challenges of the Metastatic Cascade.Frontiers in bioengineering and biotechnology · 2021Review
- Digitally Fabricated and Naturally Augmented In Vitro Tissues.Advanced healthcare materials · 2021Review
- Engineered fluidic systems to understand lymphatic cancer metastasis.Biomicrofluidics · 2020Review
- A 3D Printed Hanging Drop Dripper for Tumor Spheroids Analysis Without Recovery.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the push to reduce in vivo approaches, the demand for microphysiological models that recapitulate the in vivo settings in vitro is dramatically increasing. Here, we present an extracellular matrix-integrated microfluidic chip with a rounded microvessel of ~100 µm in diameter. Our system displays favorable characteristics for broad user adaptation: simplified procedure for vessel creation, minimised use of reagents and cells, and the ability to couple live-cell imaging and image analysis to study dynamics of cell-microenvironment interactions in 3D. Using this platform, the dynamic process of single breast cancer cells (LM2-4175) exiting the vessel lumen into the surrounding extracellular matrix was tracked. Here, we show that the presence of endothelial lining significantly reduced the cancer exit events over the 15-hour imaging period: there were either no cancer cells exiting, or the fraction of spontaneous exits was positively correlated with the number of cancer cells in proximity to the endothelial barrier. The capability to map the z-position of individual cancer cells within a 3D vessel lumen enabled us to observe cancer cell transmigration 'hot spot' dynamically. We also suggest the variations in the microvessel qualities may lead to the two distinct types of cancer transmigration behaviour. Our findings provide a tractable in vitro model applicable to other areas of microvascular research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.