Evidence map›Paper›PMID 30126376›Full record

ArticleBMC cancer2018

Malignant canine mammary epithelial cells shed exosomes containing differentially expressed microRNA that regulate oncogenic networks.

Eric J Fish, Kristopher J Irizarry, Patricia DeInnocentes, Connor J Ellis, Nripesh Prasad, Anthony G Moss, R Curt Bird

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 50 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Eric J FishDepartment of Pathobiology, College of Veterinary Medicine, Auburn University, 166 Greene Hall, Auburn, AL, 36849, USA. EJF0007@auburn.edu.ORCID http://orcid.org/0000-0002-4232-6143
Kristopher J IrizarryCollege of Veterinary Medicine, Western University of Health Sciences, Pomona, CA, 91766, USA.
Patricia DeInnocentesDepartment of Pathobiology, College of Veterinary Medicine, Auburn University, 166 Greene Hall, Auburn, AL, 36849, USA.
Connor J EllisCollege of Veterinary Medicine, Western University of Health Sciences, Pomona, CA, 91766, USA.
Nripesh PrasadGenomic Services Laboratory, Hudson Alpha Institute for Biotechnology, Huntsville, AL, 35806, USA.
Anthony G MossDepartment of Biology, College of Science and Mathematics, Auburn University, Auburn, AL, 36849, USA.
R Curt BirdDepartment of Pathobiology, College of Veterinary Medicine, Auburn University, 166 Greene Hall, Auburn, AL, 36849, USA.
Auburn University · USWestern University of Health Sciences · USHudsonAlpha Institute for Biotechnology · US

Funding

Scott-Ritchey Research Center SRRC-2015-02
6 · The paper itself

Abstract

backgroundBreast (mammary) cancers in human (BC) and canine (CMT) patients share clinical, pathological, and molecular similarities that suggest dogs may be a useful translational model. Many cancers, including BC, shed exosomes that contain microRNAs (miRs) into the microenvironment and circulation, and these may represent biomarkers of metastasis and tumor phenotype.

methodsThree normal canine mammary epithelial cell (CMEC) cultures and 5 CMT cell lines were grown in serum-free media. Exosomes were isolated from culture media by ultracentrifugation then profiled by transmission electron microscopy, dynamic light scattering, and Western blot. Exosomal small RNA was deep-sequenced on an Illumina HiSeq2500 sequencer and validated by qRT-PCR. In silico bioinformatic analysis was carried out to determine microRNA gene and pathway targets.

resultsCMEC and CMT cell lines shed round, "cup-shaped" exosomes approximately 150-200 nm, and were immunopositive for exosomal marker CD9. Deep-sequencing averaged ~ 15 million reads/sample. Three hundred thirty-eight unique miRs were detected, with 145 having > ±1.5-fold difference between one or more CMT and CMEC samples. Gene ontology analysis revealed that the upregulated miRs in this exosomal population regulate a number of relevant oncogenic networks. Several miRNAs including miR-18a, miR-19a and miR-181a were predicted in silico to target the canine estrogen receptor (ESR1α).

conclusionsCMEC and CMT cells shed exosomes in vitro that contain differentially expressed miRs. CMT exosomal RNA expresses a limited number of miRs that are up-regulated relative to CMEC, and these are predicted to target biologically relevant hormone receptors and oncogenic pathways. These results may inform future studies of circulating exosomes and the utility of miRs as biomarkers of breast cancer in women and dogs.

Indexed as

AnimalsBreast NeoplasmsCarcinogenesisCell Line, TumorDisease Models, AnimalDogsEpithelial CellsEstrogen Receptor alphaExosomesFemaleGene Expression Regulation, NeoplasticGene Regulatory NetworksHigh-Throughput Nucleotide SequencingHumansMammary Neoplasms, AnimalMicroRNAsEstrogen Receptor alphaMicroRNAsBioinformaticsCanineDeep-sequencingEstrogen receptorExosomalExosomeMammary cancermicroRNAmiR-18aRNAseqTranslational

Identifiers

PMID30126376
PMCPMC6102898
OpenAlexW2888277493

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.