Evidence map›Paper›PMID 30126249›Full record

ArticleInternational journal of molecular sciences2018

Expression, Functional Characterization, and Preliminary Crystallization of the Cochaperone Prefoldin from the Thermophilic Fungus

Kento Morita, Yohei Y Yamamoto, Ayaka Hori, Tomohiro Obata, Yuko Uno, Kyosuke Shinohara, Keiichi Noguchi, Kentaro Noi, Teru Ogura, Kentaro Ishii and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 2 countries.

Kento MoritaDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka, Koganei, Tokyo 184-8588, Japan. kento.morita@yohda.net.
Yohei Y YamamotoDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka, Koganei, Tokyo 184-8588, Japan. yohei.yamamoto@yohda.net.
Ayaka HoriDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka, Koganei, Tokyo 184-8588, Japan. ayaka.hori@yohda.net.
Tomohiro ObataDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka, Koganei, Tokyo 184-8588, Japan. tomohiro.obata@yohda.net.
Yuko UnoDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka, Koganei, Tokyo 184-8588, Japan. yuko.uno@yohda.net.
Kyosuke ShinoharaDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka, Koganei, Tokyo 184-8588, Japan. k_shino@cc.tuat.ac.jp.
Keiichi NoguchiInstrumentation Analysis Center, Tokyo University of Agriculture and Technology, Naka, Koganei, Tokyo 184-8588, Japan. knoguchi@cc.tuat.ac.jp.
Kentaro NoiDepartment of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 860-0811, Japan.. noi@abc.me.es.osaka-u.ac.jp.
Teru OguraDepartment of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 860-0811, Japan.. ogura@gpo.kumamoto-u.ac.jp.
Kentaro IshiiExploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Myodaiji, Okazaki 444-8787, Japan. ishii@ims.ac.jp.
Koichi KatoExploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Myodaiji, Okazaki 444-8787, Japan. kkatonmr@ims.ac.jp.ORCID 0000-0002-0847-7511
Mahito KikumotoStructural Biology Research Center, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya, Aichi 464-8601, Japan. kikumoto.mahito@a.mbox.nagoya-u.ac.jp.
Rocio ArranzDepartamento de Estructura de Macromoléculas, Centro Nacional de Biotecnología (CNB-CSIC), Madrid 28049, Spain. rarranz@cnb.csic.es.
Jose M ValpuestaDepartamento de Estructura de Macromoléculas, Centro Nacional de Biotecnología (CNB-CSIC), Madrid 28049, Spain. jmv@cnb.csic.es.ORCID 0000-0001-7468-8053
Masafumi YohdaDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka, Koganei, Tokyo 184-8588, Japan. yohda@cc.tuat.ac.jp.
Tokyo University of Agriculture and Technology · JPCentro Nacional de Biotecnología · ESJapan Science and Technology Agency · JPNational Institutes of Natural Sciences · JPNagoya University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Prefoldin is a hexameric molecular chaperone found in the cytosol of archaea and eukaryotes. Its hexameric complex is built from two related classes of subunits, and has the appearance of a jellyfish: Its body consists of a double β-barrel assembly with six long tentacle-like coiled coils protruding from it. Using the tentacles, prefoldin captures an unfolded protein substrate and transfers it to a group II chaperonin. Based on structural information from archaeal prefoldins, mechanisms of substrate recognition and prefoldin-chaperonin cooperation have been investigated. In contrast, the structure and mechanisms of eukaryotic prefoldins remain unknown. In this study, we succeeded in obtaining recombinant prefoldin from a thermophilic fungus,

Indexed as

AnimalsChaetomiumChickensCloning, MolecularCrystallizationFungal ProteinsGene ExpressionModels, MolecularMolecular ChaperonesProtein AggregatesProtein BindingProtein FoldingRecombinant ProteinsSubstrate SpecificitySwineFungal ProteinsMolecular ChaperonesprefoldinProtein AggregatesRecombinant ProteinsChaetomium thermophilumchaperonechaperoninfoldinginteractionproteostasis

Identifiers

PMID30126249
PMCPMC6121465
OpenAlexW2886688080

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.