ArticleProceedings of the National Academy of Sciences of the United States of America1986
Sequential expression of protooncogenes during lectin-stimulated mitogenesis of normal human lymphocytes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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Who cites it
99 citing papers in PubMed, 335 citations in OpenAlex.
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- Immunomodulatory Function of the Tumor Suppressor p53 in Host Immune Response and the Tumor Microenvironment.International journal of molecular sciences · 2016Review
- New perspective on targeting the tumor suppressor p53 pathway in the tumor microenvironment to enhance the efficacy of immunotherapy.Journal for immunotherapy of cancer · 2015Article
- Homeostatic and innate immune responses: role of the transmembrane glycoprotein CD98.Cellular and molecular life sciences : CMLS · 2012Review
- Transcriptional Regulation of the p53 Tumor Suppressor Gene in S-Phase of the Cell-Cycle and the Cellular Response to DNA Damage.Biochemistry research international · 2012Article
- A role for the immediate early gene product c-fos in imprinting T cells with short-term memory for signal summation.PloS one · 2011Article
- Gene expression profiles in human peripheral blood mononuclear cells as biomarkers for nutritional in vitro and in vivo investigations.Genes & nutrition · 2010Article
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- c-Myb contributes to G2/M cell cycle transition in human hematopoietic cells by direct regulation of cyclin B1 expression.Molecular and cellular biology · 2007Article
- Increased p53 transcription prior to DNA synthesis is regulated through a novel regulatory element within the p53 promoter.Oncogene · 2006Article
- Telomerase reverse transcriptase gene is a direct target of c-Myc but is not functionally equivalent in cellular transformation.Oncogene · 1999Article
- Pharmacological approaches to the prevention of restenosis after coronary angioplasty.Drugs & aging · 1998Review
- Cytokine gene expression in normal human lymphocytes in response to stimulation.Clinical and diagnostic laboratory immunology · 1998Article
- D-type cyclins repress transcriptional activation by the v-Myb but not the c-Myb DNA-binding domain.The EMBO journal · 1998Article
- Resistance to apoptosis in CTLL-2 cells constitutively expressing c-Myb is associated with induction of BCL-2 expression and Myb-dependent regulation of bcl-2 promoter activity.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Similarity of apoptosis induction by 2-chlorodeoxyadenosine and cisplatin in human mononuclear blood cells.British journal of cancer · 1997Article
- Identification of an inducible regulator of c-myb expression during T-cell activation.Molecular and cellular biology · 1996Article
39 more citing papers are in PubMed but not listed here.
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4 authors at 1 institution in 1 country.
Funding
Abstract
The proliferation of non-neoplastic T lymphocytes is regulated, in part, by the coordinated expression of genes encoding T-cell growth factor (interleukin 2, IL2), IL2 receptors (IL2R), and transferrin receptors (TFR). In addition to growth factors and their receptors, protooncogenes may regulate lymphocyte proliferation. We used cloned cDNAs homologous to 21 different protooncogenes to screen for their expression at the mRNA level in human peripheral blood mononuclear cells (PBMC) stimulated with the mitogenic lectin phytohemagglutinin (PHA), and we compared the time course of accumulation of mRNAs for these protooncogenes to that of mRNAs for the IL2, IL2R, TFR, and histone H3 genes. mRNAs for c-abl, c-ets, c-yes, and N-ras were present in unstimulated PBMC. After stimulation of PBMC by PHA, we detected marked increases within 10 min in the levels of mRNA for c-fos and c-myc; within 6 hr for IL2 and IL2R mRNAs; within 14 hr for c-myb, p53, N-ras, and TFR mRNAs; and within 24-36 hr for H3 mRNA. Expression of c-abl, c-ets, and c-yes increased gradually following stimulation with PHA. None of the other protooncogenes tested was expressed in PBMC. Addition of the protein synthesis inhibitor cycloheximide, before the addition of PHA to cultures, abolished the PHA-induced accumulation of mRNAs for c-myb, N-ras, and TFR, but not of mRNAs for c-fos, c-myc, IL2, and IL2R. These data indicate that c-fos, c-myc, IL2, and IL2R belong to a group of genes expressed early, whereas c-myb, N-ras, and TFR belong to a group of genes expressed later in PHA-activated PBMC, and that the products of the c-fos and c-myc protooncogenes are not required for expression of IL2 or IL2R genes. Addition of purified IL2 augmented the expression of the later-expressed genes c-myb, p53, N-ras, and TFR in PHA-stimulated cultures of PBMC, as well as of the early genes c-myc and IL2R, but not of c-fos and IL2, thus suggesting that PHA and IL2 stimulate the expression of overlapping, but nonidentical, sets of genes in PBMC.
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