ArticleJournal of experimental botany2018
RNAi-mediated suppression of three carotenoid-cleavage dioxygenase genes, OsCCD1, 4a, and 4b, increases carotenoid content in rice.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- An evolutionarily diverged CCD4 enzyme negatively regulates mesocotyl elongation in rice.The New phytologist · 2026Article
- Mangrove-Derived Endophytic Bacteria Enhance Growth, Yield, and Stress Resilience in Rice.International journal of molecular sciences · 2025Article
- Review
- A detailed comparative in silico and functional analysis of ccd1 gene in maize gives new insights of its expression and functions.Molecular biology reports · 2025Article
- Identifying molecular targets for modulating carotenoid accumulation in rice grains.Biochemistry and biophysics reports · 2024Article
- Advances in RNA Interference for Plant Functional Genomics: Unveiling Traits, Mechanisms, and Future Directions.Applied biochemistry and biotechnology · 2024Review
- Physicochemical Properties and Antioxidant Activity of CRISPR/Cas9-Edited TomatoInternational journal of molecular sciences · 2024Article
- A Frameshift Mutation in the Mg-Chelatase I Subunit GenePlants (Basel, Switzerland) · 2023Article
- Physiological changes of microalga Dunaliella parva under the treatment of PEG, CaCl2.PloS one · 2023Article
- Mutant combinations of lycopene ɛ-cyclase and β-carotene hydroxylase 2 homoeologs increased β-carotene accumulation in endosperm of tetraploid wheat (Triticum turgidum L.) grains.Plant biotechnology journal · 2022Article
- Mining of Potential Gene Resources for Breeding Nutritionally Improved Maize.Plants (Basel, Switzerland) · 2022Review
- Identification of the carotenoid cleavage dioxygenase genes and functional analysis revealFrontiers in plant science · 2022Article
- Differential Regulation of an OsIspH1, the Functional 4-Hydroxy-3-Methylbut-2-Enyl Diphosphate Reductase, for Photosynthetic Pigment Biosynthesis in Rice Leaves and Seeds.Frontiers in plant science · 2022Article
- IdentifyingFrontiers in plant science · 2022Article
- Overexpression ofFrontiers in plant science · 2022Article
- Carotenoid cleavage dioxygenases (HD-CCD1A and B) contribute as strong negative regulators of β-carotene in Indian bread wheat (cv. HD2967).3 Biotech · 2021Article
- Biofortification of Cereals and Pulses Using New Breeding Techniques: Current and Future Perspectives.Frontiers in nutrition · 2021Review
- Carotenoids in Cereal Food Crops: Composition and Retention throughout Grain Storage and Food Processing.Plants (Basel, Switzerland) · 2019Review
- Carotenoid Cleavage Dioxygenases: Identification, Expression, and Evolutionary Analysis of This Gene Family in Tobacco.International journal of molecular sciences · 2019Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carotenoids of staple food crops have a high nutritional value as provitamin A components in the daily diet. To increase the levels of carotenoids, inhibition of carotenoid-cleavage dioxygenases (CCDs), which degrade carotenoids, has been considered as a promising target in crop biotechnology. In this study, suppression of the OsCCD1, OsCCD4a, and OsCCD4b genes using RNAi was verified in transgenic rice plants by quantitative RT-PCR and small RNA detection. Leaf carotenoids were significantly increased overall in OsCCD4a-RNAi lines of the T1 generation, and the highest accumulation of 1.3-fold relative to non-transgenic plants was found in a line of the T2 generation. The effects on seed carotenoids were determined via cross-fertilization between β-carotene-producing transgenic rice and one of two independent homozygous lines of OsCCD1-RNAi, OsCCD4a-RNAi, or OsCCD4b-RNAi. This showed that carotenoids were increased to a maximum of 1.4- and 1.6-fold in OsCCD1-RNAi and OsCCD4a-RNAi, respectively, with a different preference toward α-ring and β-ring carotenoids; levels could not be established in OsCCD4b-RNAi. In addition, the contents of four carotenoids decreased when OsCCD1, OsCCD4a, and OsCCD4b were overexpressed in E. coli strains accumulating phytoene, lycopene, β-carotene, and zeaxanthin. OsCCD1 and OsCCD4a had a similar high carotenoid degrading activity, followed by OsCCD4b without substrate specificity. Overall, our results suggest that suppresing OsCCD4a activity may have potential as a tool for enhancing the carotenoid content of seed endosperms and leaves in rice.
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