ArticlePorcine health management2018
An efficiency comparison of different in vitro fertilization methods: IVF, ICSI, and PICSI for embryo development to the blastocyst stage from vitrified porcine immature oocytes.
Article in Porcine health management, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 55 citations in OpenAlex.
- Impact of Methylparaben on Cumulus Cell DNA Integrity and Porcine Oocyte Developmental Competence In Vitro.Journal of applied toxicology : JAT · 2026Article
- Vitrification altered αV and β3 integrin expression in mouse oocytes in a maturation stage-dependent manner.Molecular biology reports · 2026Article
- Indications for Micro-Injection of Sperm in Assisted Reproduction: A Consensus Statement.The Australian & New Zealand journal of obstetrics & gynaecology · 2026Article
- Factors affecting the intracytoplasmic sperm cell injection outcomes: A meta-analysis of porcine studies.Journal of advanced research · 2025Article
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- Hydrogel technologies in andrology: advances in research and prospective applications.Frontiers in pharmacology · 2025Review
- The Relationship Between Smooth Endoplasmic Reticulum Clusters in Metaphase II Oocytes and Embryological and Birth Outcomes in Infertile Couples.International journal of general medicine · 2024Article
- New Strategies for Conservation of Gentile di Puglia Sheep Breed, an Autochthonous Capital of Millennial Tradition in Southern Italy.Animals : an open access journal from MDPI · 2023Article
- Fluorescent nanodiamond labels: Size and concentration matters for sperm cell viability.Materials today. Bio · 2023Article
- Influence of a hyaluronan-binding system for sperm selection in intracytoplasmic sperm injection cycles on embryo morphokinetic parameters and in vitro fertilization cycle outcomes.Archives of gynecology and obstetrics · 2023Article
- Sperm selection by the oviduct: perspectives for male fertility and assisted reproductive technologies†.Biology of reproduction · 2023Article
- Antioxidant procyanidin B2 protects oocytes against cryoinjuries via mitochondria regulated cortical tension.Journal of animal science and biotechnology · 2022Article
- Effect of Sperm 26S Proteasome on Embryo Formation and Development in In Vitro Fertilization.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- Investigation of the Efficacy of Dithiothreitol and Glutathione on In Vitro Fertilization of Cryopreserved Large White Boar Semen.Animals : an open access journal from MDPI · 2022Article
- Unveiling how vitrification affects the porcine blastocyst: clues from a transcriptomic study.Journal of animal science and biotechnology · 2022Article
- Glycine and Melatonin Improve Preimplantation Development of Porcine Oocytes Vitrified at the Germinal Vesicle Stage.Frontiers in cell and developmental biology · 2022Article
- DNA damage in cumulus cells generated after the vitrification of in vitro matured porcine oocytes and its impact on fertilization and embryo development.Porcine health management · 2021Article
- Review
- Chronic Stress Detrimentally Affects In Vivo Maturation in Rat Oocytes and Oocyte Viability at All Phases of the Estrous Cycle.Animals : an open access journal from MDPI · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMost studies carried out to evaluate recovery and development after porcine oocyte vitrification, reported better rates when cryopreserved in embryonic development stages or zygotes, but not in immature oocytes. For this reason, many studies are performed to improve immature oocyte vitrification protocols testing the use of different cryoprotectant concentrations, cooling devices, incubation times; but only a few of them have evaluated which fertilization procedure enhances blastocyst rates in vitrified oocytes. Therefore, this study was aimed to evaluate: 1) if the sperm selection with hyaluronic acid (HA) or polyvinylpyrrolidone (PVP) before injection could play a key role in increasing fertilization and blastocyst formation and 2) the embryo developmental ability and blastocyst production of porcine immature oocytes retrieved after vitrification-warming and co-cultured with granulosa cells during IVM, using different fertilization techniques: in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI) and conventional ICSI with hyaluronic acid (HA) sperm selection, known as physiological intracytoplasmic sperm injection (PICSI) and.
resultsSperm selected with HA-PICSI displayed a higher percentage of live/acrosome reacted status compared to those in control and exposed to PVP. Higher dead/acrosome reacted rates were obtained after PVP exposure compared to control and HA. In oocytes, viability significantly decreased after IVM in vitrified oocytes. Besides, IVM rates were not different between control denuded oocytes cultured with granulosa cells (DO-GC) and vitrified oocytes. Regarding fertilization parameters, IVF showed higher percentages of total fertilization rate than those obtained by ICSI and PICSI. However, results demonstrate that PICSI fertilization increased the blastocysts formation rate in control DO-GC and vitrified oocytes compared to IVF and ICSI.
conclusionsTo achieve high blastocyst formation rates from vitrified GV oocytes, it is recommended that sperm should be selected with HA instead of PVP before injection since high viability and acrosome reaction rates were obtained. Also, PICSI fertilization was the best method to produce higher blastocyst rates compared to the IVF and ICSI procedures.
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