ArticleDevelopmental biology2018
CBD-1 organizes two independent complexes required for eggshell vitelline layer formation and egg activation in C. elegans.
Article in Developmental biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed.
- Integrative Quantitative Proteomics and N-Glycoproteomics Reveal Age-Processing of N-Glycosylation in Caenorhabditis elegans.Proteomics · 2026Article
- Co-Option and Conflict: The Deep Evolutionary History of ZP-Domain Proteins from ECMs to Species Barriers.International journal of molecular sciences · 2026Review
- Functional requirement for Dicer helicase arginine methylation in 26 G siRNA biogenesis and oocyte meiotic program.Nature communications · 2026Article
- Oocyte surface proteins EGG-1 and EGG-2 are required for eggshell integrity in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2026Article
- Article
- Oocyte surface proteins EGG-1 and EGG-2 are required for eggshell integrity inbioRxiv : the preprint server for biology · 2025Article
- A sperm-oocyte protein partnership required for egg activation in Caenorhabditis elegans.Development (Cambridge, England) · 2025Article
- Simple In-Cell Processing Enables Deep Proteome Analysis of Low-InputAnalytical chemistry · 2025Article
- Mechanical force of uterine occupation enables large vesicle extrusion from proteostressed maternal neurons.eLife · 2024Article
- CED-5/CED-12 (DOCK/ELMO) can promote and inhibit F-actin formation via distinct motifs that may target different GTPases.PLoS genetics · 2024Article
- Nuclear receptor signaling via NHR-49/MDT-15 regulates stress resilience and proteostasis in response to reproductive and metabolic cues.Genes & development · 2024Article
- Mechanical force of uterine occupation enables large vesicle extrusion from proteostressed maternal neurons.bioRxiv : the preprint server for biology · 2024Article
- Transcriptomic Analysis of the Spatiotemporal Axis of Oogenesis and Fertilization inbioRxiv : the preprint server for biology · 2024Article
- MARC-3, a membrane-associated ubiquitin ligase, is required for fast polyspermy block in Caenorhabditis elegans.Nature communications · 2024Article
- Transcriptomic analysis of the spatiotemporal axis of oogenesis and fertilization inFrontiers in cell and developmental biology · 2024Article
- CED-5/CED-12 (DOCK/ELMO) can promote and inhibit F-actin formation via distinct motifs that target different GTPases.bioRxiv : the preprint server for biology · 2023Article
- The effects of different biochars on Caenorhabditis elegans and the underlying transcriptomic mechanisms.PloS one · 2023Article
- Disruption ofmicroPublication biology · 2022Article
- Loss of the seipin gene perturbs eggshell formation inDevelopment (Cambridge, England) · 2020Article
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Abstract
Metazoan eggs have a specialized coat of extracellular matrix that aids in sperm-egg recognition. The coat is rapidly remodeled after fertilization to prevent polyspermy and establish a more permanent barrier to protect the developing embryo. In nematodes, this coat is called the vitelline layer, which is remodeled into the outermost layer of a rigid and impermeable eggshell. We have identified three key components of the vitelline layer structural scaffold - PERM-2, PERM-4 and CBD-1, the first such proteins to be described in the nematode C. elegans. CBD-1 tethered PERM-2 and PERM-4 to the nascent vitelline layer via two N-terminal chitin-binding domains. After fertilization, all three proteins redistributed from the zygote surface to the outer eggshell. Depletion of PERM-2 and PERM-4 from the scaffold led to a porous vitelline layer that permitted soluble factors to leak through the eggshell and resulted in embryonic death. In addition to its role in vitelline layer assembly, CBD-1 is also known to anchor a protein complex required for fertilization and egg activation (EGG-1-5/CHS-1/MBK-2). We found the PERM complex and EGG complex to be functionally independent, and structurally organized through distinct domains of CBD-1. CBD-1 is thus a multifaceted regulator that promotes distinct aspects of vitelline layer assembly and egg activation. In sum, our findings characterize the first vitelline layer components in nematodes, and provide a foundation through which to explore both conserved and species-specific strategies used by animals to build protective barriers following fertilization.
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