ArticlePlant biotechnology journal2019
Expression of the Arabidopsis thaliana immune receptor EFR in Medicago truncatula reduces infection by a root pathogenic bacterium, but not nitrogen-fixing rhizobial symbiosis.
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- Root colonization by beneficial rhizobacteria.FEMS microbiology reviews · 2024Pooled it
- A Specific Sinorhizobium Flagellin Suppresses Legume Nodulation Through Immune Activation.Plant biotechnology journal · 2026Article
- Heterologous expression of Arabidopsis FLS2 in Nicotiana benthamiana confers responsiveness to Ralstonia flg22 and activation of anti-bacterial immunity.Plant biotechnology journal · 2025Article
- The receptor-like protein RXEG1 confers broad-spectrum resistance in soybean and cotton by recognizing microbial glycoside hydrolase 12 proteins.Plant biotechnology journal · 2025Article
- Harnessing the Potential of CRISPR/Cas in Targeted Alfalfa Improvement for Stress Resilience.International journal of molecular sciences · 2025Review
- Plant pattern recognition receptors: from evolutionary insight to engineering.Nature reviews. Genetics · 2025Review
- All Roads Lead to Rome: Pathways to Engineering Disease Resistance in Plants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Transposon-triggered epigenetic chromatin dynamics modulate EFR-related pathogen response.Nature structural & molecular biology · 2025Article
- The perception and evolution of flagellin, cold shock protein and elongation factor Tu from vector-borne bacterial plant pathogens.Molecular plant pathology · 2024Article
- The Emerging Role ofPlants (Basel, Switzerland) · 2024Review
- Recognition of glycoside hydrolase 12 proteins by the immune receptor RXEG1 confers Fusarium head blight resistance in wheat.Plant biotechnology journal · 2023Article
- Legumes Regulate Symbiosis with Rhizobia via Their Innate Immune System.International journal of molecular sciences · 2023Review
- Article
- Engineering plant immune circuit: walking to the bright future with a novel toolbox.Plant biotechnology journal · 2023Review
- Highly Efficient Gene Knockout in Medicago truncatula Genotype R108 Using CRISPR-Cas9 System and an Optimized Agrobacterium Transformation Method.Methods in molecular biology (Clifton, N.J.) · 2023Article
- PairedProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Transgenic Improvement for Biotic Resistance of Crops.International journal of molecular sciences · 2022Review
- Evolutionary gain and loss of a plant pattern-recognition receptor for HAMP recognition.eLife · 2022Article
- Receptor-like Kinases (LRR-RLKs) in Response of Plants to Biotic and Abiotic Stresses.Plants (Basel, Switzerland) · 2022Review
- Research Progress and Prospect of Alfalfa Resistance to Pathogens and Pests.Plants (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
Interfamily transfer of plant pattern recognition receptors (PRRs) represents a promising biotechnological approach to engineer broad-spectrum, and potentially durable, disease resistance in crops. It is however unclear whether new recognition specificities to given pathogen-associated molecular patterns (PAMPs) affect the interaction of the recipient plant with beneficial microbes. To test this in a direct reductionist approach, we transferred the Brassicaceae-specific PRR ELONGATION FACTOR-THERMO UNSTABLE RECEPTOR (EFR), conferring recognition of the bacterial EF-Tu protein, from Arabidopsis thaliana to the legume Medicago truncatula. Constitutive EFR expression led to EFR accumulation and activation of immune responses upon treatment with the EF-Tu-derived elf18 peptide in leaves and roots. The interaction of M. truncatula with the bacterial symbiont Sinorhizobium meliloti is characterized by the formation of root nodules that fix atmospheric nitrogen. Although nodule numbers were slightly reduced at an early stage of the infection in EFR-Medicago when compared to control lines, nodulation was similar in all lines at later stages. Furthermore, nodule colonization by rhizobia, and nitrogen fixation were not compromised by EFR expression. Importantly, the M. truncatula lines expressing EFR were substantially more resistant to the root bacterial pathogen Ralstonia solanacearum. Our data suggest that the transfer of EFR to M. truncatula does not impede root nodule symbiosis, but has a positive impact on disease resistance against a bacterial pathogen. In addition, our results indicate that Rhizobium can either avoid PAMP recognition during the infection process, or is able to actively suppress immune signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.