Evidence map›Paper›PMID 30120856›Full record

ArticleParasite immunology2018

Use of VHH antibodies for the development of antigen detection test for visceral leishmaniasis.

Claudia Abeijon, Julia Dilo, Jacqueline M Tremblay, Agostinho G Viana, Lilian L Bueno, Silvio F G Carvalho, Ricardo T Fujiwara, Charles B Shoemaker, Antonio Campos-Neto

Open access · hybridAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasite immunology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 14 citations in OpenAlex.

  1. Pooled it
  2. Nanobodies in animal infectious disease control: diagnosis and therapy.Frontiers in cellular and infection microbiology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Claudia AbeijonDetectoGen Inc., Grafton, Massachusetts.
Julia DiloDetectoGen Inc., Grafton, Massachusetts.
Jacqueline M TremblayCummings School of Veterinary Medicine at Tufts University, North Grafton, Massachusetts.
Agostinho G VianaFederal University of Minas Gerais, Belo Horizonte, Brazil.
Lilian L BuenoFederal University of Minas Gerais, Belo Horizonte, Brazil.
Silvio F G CarvalhoState University of Montes Claros, Montes Claros, Brazil.
Ricardo T FujiwaraFederal University of Minas Gerais, Belo Horizonte, Brazil.ORCID http://orcid.org/0000-0002-4713-575X
Charles B ShoemakerCummings School of Veterinary Medicine at Tufts University, North Grafton, Massachusetts.
Antonio Campos-NetoDetectoGen Inc., Grafton, Massachusetts.ORCID http://orcid.org/0000-0003-1355-2798
Tufts University · USUniversidade Federal de Minas Gerais · BRNew England Disabled Sports · USUniversidade Estadual de Montes Claros · BR

Funding

Novel non-invasive antigen detection assay for the diagnosis of active visceral leishmaniasis and to monitor the therapy efficacy of this diseaseR44AI113992 · NIAID · DETECTOGEN, INC. · PI ABEIJON, CLAUDIA · 2017 to 2019
$1.9M
Development of a protein-based antigen detection test for kala-azarR43AI113992 · NIAID · DETECTOGEN, INC. · PI ABEIJON, CLAUDIA · 2015 to 2016
$420k
Massachusetts Life Sciences CenterNIAID NIH HHS R43 AI113992NIAID NIH HHS R44 AI113992NIH HHS R44-AI113992
6 · The paper itself

Abstract

We have recently developed a sensitive and specific urine-based antigen detection ELISA for the diagnosis of visceral leishmaniasis (VL). This assay used rabbit IgG and chicken IgY polyclonal antibodies specific for the Leishmania infantum proteins iron superoxide dismutase 1 (Li-isd1), tryparedoxin1 (Li-txn1) and nuclear transport factor 2 (Li-ntf2). However, polyclonal antibodies have limitations for upscaling and continuous supply. To circumvent these hurdles, we began to develop immortalized monoclonal antibodies. We opted for recombinant camelid VHHs because the technology for their production is well established and they do not have Fc, hence providing less ELISA background noise. We report here an assay development using VHHs specific for Li-isd1 and Li-ntf2. This new assay was specific and had analytical sensitivity of 15-45 pg/mL of urine. The clinical sensitivity was comparable to that obtained with the ELISA assembled with conventional rabbit and chicken antibodies to detect these two antigens. Therefore, similar to our former studies with conventional antibodies, the future inclusion of VHH specific for Li-txn1 and/or other antigens should further increase the sensitivity of the assay. These results confirm that immortalized VHHs can replace conventional antibodies for the development of an accurate and reproducible antigen detection diagnostic test for VL.

Indexed as

AdolescentAdultAnimalsAntibodies, ProtozoanAntigens, ProtozoanCamelids, New WorldChickensChildChild, PreschoolFemaleHumansImmunologic TestsInfantLeishmania infantumLeishmaniasis, VisceralMaleAntibodies, ProtozoanAntigens, ProtozoanSingle-Domain Antibodies

Identifiers

PMID30120856
PMCPMC6220836
OpenAlexW2886736600

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.