ArticleScientific reports2018
Development of an oxidative stress in vitro assay in zebrafish (Danio rerio) cell lines.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Toxicity testing of pesticides in zebrafish-a systematic review on chemicals and associated toxicological endpoints.Environmental science and pollution research international · 2020Pooled it
- Human and zebrafish cross-species comparison on ligand affinity towards the aryl hydrocarbon receptor and Keap1 oxidative stress sensor: a molecular docking dataset.Data in brief · 2026Article
- A dataset on the multi-species and multi-endpoint bioanalytical assessment of environmental samples derived from Swedish wastewater treatment plants.Data in brief · 2026Article
- Multiomics profiling of zebrafish embryonic cell line PAC2 across growth phases to assess its relevance for toxicological studies.PloS one · 2026Article
- Evaluation of Oxidative Stress and Antioxidant Effects of Methylxanthines in Adult Zebrafish Exposed to Zinc Oxide Nanoparticles (ZnO-NPs).Medicina (Kaunas, Lithuania) · 2025Article
- Comparative Evaluation of Toxicity End Points in Unicellular Bioassays for Environmental Pollutant Assessment.Environmental science & technology · 2025Article
- Exploring Oxidative Stress Mechanisms of Nanoparticles Using Zebrafish (Antioxidants (Basel, Switzerland) · 2025Review
- Diazinon induces testicular dysfunction and testicular cell damage through increased reactive oxygen species production in mouse.Cell death discovery · 2025Article
- Development, scrutiny, and modulation of transient reporter gene assays of the xenobiotic metabolism pathway in zebrafish hepatocytes.Cell biology and toxicology · 2023Article
- Transfection of Sponge Cells and Intracellular Localization of Cancer-Related MYC, RRAS2, and DRG1 Proteins.Marine drugs · 2023Article
- Mechanisms of Silver Nanoparticle Uptake by Embryonic Zebrafish Cells.Nanomaterials (Basel, Switzerland) · 2021Article
- Modeling Bioavailable Concentrations in Zebrafish Cell Lines and Embryos Increases the Correlation of Toxicity Potencies across Test Systems.Environmental science & technology · 2021Article
- Potentials and pitfalls of transient in vitro reporter bioassays: interference by vector geometry and cytotoxicity in recombinant zebrafish cell lines.Archives of toxicology · 2020Article
- Redox toxicology of environmental chemicals causing oxidative stress.Redox biology · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The nuclear factor erythroid 2-related factor 2 (Nrf2) is a key regulator of cellular defense against oxidative stress and correlated with classical toxicological endpoints. In vitro methods using fish cell lines for the assessment of aquatic toxicity are needed for mechanistic studies and as an alternative to in vivo. We describe an in vitro assay to study oxidative stress using zebrafish cell lines. Transfection efficiency of twelve commercially available transfection reagents were tested in the zebrafish cell lines ZFL, ZF4, and Pac2. The most efficient reagent for each cell line was selected for further experiments. Cells were transiently transfected with an Nrf2-responsive luciferase plasmid. The assay was tested using the oxidative stress inducing chemicals tertbutylhydroquinone, hydrogen peroxide, and sulforaphane. Of the transfected cell lines, ZF4 and ZFL showed higher sensitivity. The latter were used to study potential oxidative stress induced by pesticides (diazinon, deltamethrin, atrazine, metazachlor, terbutylazine, diuron). Besides known inducers, Nrf2 activity was also significantly induced by diazinon, deltametrin, diuron, and metazachlor. Activation of Nrf2 by metazachlor is a novel finding. The described assay could be a valuable tool for research in toxicology to study the stress response of both pure chemicals and environmental water samples.
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