ArticleScientific reports2018
Accelerated triacylglycerol production without growth inhibition by overexpression of a glycerol-3-phosphate acyltransferase in the unicellular red alga Cyanidioschyzon merolae.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 89 citations in OpenAlex.
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- Regulatory network rewiring drives strain-specific lipid accumulation response in Chlorella sorokiniana under nutrient starvation.The Plant journal : for cell and molecular biology · 2026Article
- Optimal conditions of algal breeding using neutral beam and applying it to breed Euglena gracilis strains with improved lipid accumulation.Scientific reports · 2024Article
- Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities.Biotechnology for biofuels and bioproducts · 2024Review
- Lipid accumulation mechanism of Amphora coffeaeformis under nitrogen deprivation and its application as a feed additive in Carassius auratus aquaculture.Biotechnology for biofuels and bioproducts · 2023Article
- Engineered ketocarotenoid biosynthesis in the polyextremophilic red microalgaMetabolic engineering communications · 2023Article
- Genome-Wide Analysis of Glycerol-3-Phosphate Acyltransferase (GPAT) Family inInternational journal of molecular sciences · 2023Article
- Microalgal glycerol-3-phosphate acyltransferase role in galactolipids and high-value storage lipid biosynthesis.Plant physiology · 2023Article
- Citrate synthase from Cyanidioschyzon merolae exhibits high oxaloacetate and acetyl-CoA catalytic efficiency.Plant molecular biology · 2023Article
- Novel context-specific genome-scale modelling explores the potential of triacylglycerol production by Chlamydomonas reinhardtii.Microbial cell factories · 2023Article
- Expression of glycerol-3-phosphate acyltransferase increases non-polar lipid accumulation in Nannochloropsis oceanica.Microbial cell factories · 2023Article
- Construction of Glucose-6-Phosphate Dehydrogenase Overexpression Strain ofMarine drugs · 2022Article
- Deciphering the function and evolution of the target of rapamycin signaling pathway in microalgae.Journal of experimental botany · 2022Article
- Biochemical Properties of β-Amylase from Red Algae and Improvement of Its Thermostability through Immobilization.ACS omega · 2022Article
- Development of a stable semi-continuous lipid production system of an oleaginous Chlamydomonas sp. mutant using multi-omics profiling.Biotechnology for biofuels and bioproducts · 2022Article
- Article
- The Unicellular Red AlgaPlants (Basel, Switzerland) · 2021Review
- The oleaginous astaxanthin-producing alga Chromochloris zofingiensis: potential from production to an emerging model for studying lipid metabolism and carotenogenesis.Biotechnology for biofuels · 2021Review
- Identification of Transcription Factors and the Regulatory Genes Involved in Triacylglycerol Accumulation in the Unicellular Red AlgaPlants (Basel, Switzerland) · 2021Article
- Microalgal Biodiesel Production: Realizing the Sustainability Index.Frontiers in bioengineering and biotechnology · 2021Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microalgae accumulate triacylglycerols (TAGs), a promising feedstock for biodiesel production, under unfavorable environmental or stress conditions for their growth. Our previous analyses revealed that only transcripts of CmGPAT1 and CmGPAT2, both encoding glycerol-3-phosphate acyltransferase, were increased among fatty acid and TAG synthesis genes under TAG accumulation conditions in the red alga Cyanidioschyzon merolae. In this study, to investigate the role of these proteins in TAG accumulation in C. merolae, we constructed FLAG-fused CmGPAT1 and CmGPAT2 overexpression strains. We found that CmGPAT1 overexpression resulted in marked accumulation of TAG even under normal growth conditions, with the maximum TAG productivity increased 56.1-fold compared with the control strain, without a negative impact on algal growth. The relative fatty acid composition of 18:2 in the TAGs and the sn-1/sn-3 positions were significantly increased compared with the control strain, suggesting that CmGPAT1 had a substrate preference for 18:2. Immunoblot analysis after cell fractionation and immunostaining analysis demonstrated that CmGPAT1 localizes in the endoplasmic reticulum (ER). These results indicate that the reaction catalyzed by the ER-localized CmGPAT1 is a rate-limiting step for TAG synthesis in C. merolae, and would be a potential target for improvement of TAG productivity in microalgae.
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