ArticleScientific reports2018
Multicilin and activated E2f4 induce multiciliated cell differentiation in primary fibroblasts.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- The cell cycle variant in multiciliated cells incorporates 2 centriole biogenesis cycles.bioRxiv : the preprint server for biology · 2026Article
- McIdas localizes to centrioles and controls centriole numbers through PLK4-dependent phosphorylation.EMBO reports · 2026Article
- Article
- RBL2 represses the transcriptional activity of Multicilin to inhibit multiciliogenesis.Cell death & disease · 2024Article
- Formation and function of multiciliated cells.The Journal of cell biology · 2024Review
- Deup1 Expression Interferes with Multiciliated Differentiation.Molecules and cells · 2023Article
- Bidirectional multiciliated cell extrusion is controlled by Notch-driven basal extrusion and Piezo1-driven apical extrusion.Development (Cambridge, England) · 2023Article
- Influenza Defective Interfering Virus Promotes Multiciliated Cell Differentiation and Reduces the Inflammatory Response in Mice.Journal of virology · 2023Article
- Bidirectional multiciliated cell extrusion is controlled by Notch driven basal extrusion and Piezo 1 driven apical extrusion.bioRxiv : the preprint server for biology · 2023Article
- Outcomes of the 2019 hydrocephalus association workshop, "Driving common pathways: extending insights from posthemorrhagic hydrocephalus".Fluids and barriers of the CNS · 2023Review
- Appearing and disappearing acts of cilia.Journal of biosciences · 2023Review
- Male infertility-associated Ccdc108 regulates multiciliogenesis via the intraflagellar transport machinery.EMBO reports · 2022Article
- Emi2 enables centriole amplification during multiciliated cell differentiation.Science advances · 2022Article
- Review
- Biophysical and biochemical properties of Deup1 self-assemblies: a potential driver for deuterosome formation during multiciliogenesis.Biology open · 2021Article
- A role for Cep70 in centriole amplification in multiciliated cells.Developmental biology · 2021Article
- Aquatic models of human ciliary diseases.Genesis (New York, N.Y. : 2000) · 2021Review
- Article
- Interplay of RFX transcription factors 1, 2 and 3 in motile ciliogenesis.Nucleic acids research · 2020Article
- Transcription factor TAp73 and microRNA-449 complement each other to support multiciliogenesis.Cell death and differentiation · 2019Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Multiciliated cells (MCCs) are specialized epithelial cells that project hundreds of motile cilia. To form these cilia, MCCs differentiate by dramatically expanding centriole number, using assembly factors required for centriole duplication during the cell cycle and multiple, novel assembly sites, called the deuterosome. The small coiled-coil protein, Multicilin, acting in a complex with the E2F proteins can initiate multiciliated cell differentiation, but reportedly only in a limited range of epithelial progenitors. To examine the nature of this restricted activity, we analyzed Multicilin activity in primary mouse embryonic fibroblasts (MEFs), a cell type distant from the epithelial lineages where MCCs normally arise. We show that Multicilin transcriptional activity is markedly attenuated in MEFs, where it induces only limited centriole expansion in a small fraction of cells. We further show that this transcriptional block is largely bypassed by expressing Multicilin along with a form of E2f4 where a generic activation domain from HSV1 VP16 (E2f4VP16) is fused to the carboxy terminus. MEFs respond to Multicilin and E2f4VP16 by undergoing massive centriole expansion via the deuterosome pathway, recapitulating a temporal sequence of organelle biogenesis that occurs in epithelial progenitors during MCC differentiation. These results suggest that the pattern of organelle biogenesis occurring in differentiating MCCs is largely determined by the transcriptional changes induced by Multicilin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.