ArticleBMC genomics2018
Large plasmidome of dairy Lactococcus lactis subsp. lactis biovar diacetylactis FM03P encodes technological functions and appears highly unstable.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 33 citations in OpenAlex.
- Proteomic Analysis of Lactococcus lactis FM03 Under Prolonged Alkaline Stress.Environmental microbiology · 2026Article
- Comparative genomics and methylome profiling ofMicrobial genomics · 2026Article
- Development of the Efficient Electroporation Protocol forInternational journal of molecular sciences · 2025Article
- Lactococcal mobile genetic elements harbour a diverse phage defensome rich in restriction-modification systems.Nucleic acids research · 2025Article
- A Plasmid-Encoded Surface Polysaccharide Partly BlocksInternational journal of molecular sciences · 2025Article
- Discovery of antiphage systems in the lactococcal plasmidome.Nucleic acids research · 2024Article
- Discovering genetic determinants for cell-to-cell adhesion in two prevalent conjugative lactococcal plasmids.Current research in microbial sciences · 2024Article
- Slc11 Synapomorphy: A Conserved 3D Framework Articulating Carrier Conformation Switch.International journal of molecular sciences · 2023Article
- Control of Bacterial Phenotype and Chromosomal Gene Expression by Single Plasmids ofInternational journal of molecular sciences · 2023Article
- The Presence of Plasmids inInternational journal of molecular sciences · 2023Article
- Phenotypic and Safety Assessment of the Cheese StrainInternational journal of molecular sciences · 2022Article
- Directed Recovery and Molecular Characterization of Antibiotic Resistance Plasmids from Cheese Bacteria.International journal of molecular sciences · 2021Article
- DAIRYdb: a manually curated reference database for improved taxonomy annotation of 16S rRNA gene sequences from dairy products.BMC genomics · 2019Article
- Long-read based de novo assembly of low-complexity metagenome samples results in finished genomes and reveals insights into strain diversity and an active phage system.BMC microbiology · 2019Article
- Article
- Comparative Genomics ofFrontiers in microbiology · 2019Article
- Comparative Genome Analysis ofFrontiers in microbiology · 2019Article
- TheFrontiers in microbiology · 2019Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundImportant industrial traits have been linked to plasmids in Lactococcus lactis.
resultsThe dairy isolate L. lactis subsp. lactis biovar diacetylactis FM03P was sequenced revealing the biggest plasmidome of all completely sequenced and published L. lactis strains up till now. The 12 plasmids that were identified are: pLd1 (8277 bp), pLd2 (15,218 bp), pLd3 (4242 bp), pLd4 (12,005 bp), pLd5 (7521 bp), pLd6 (3363 bp), pLd7 (30,274 bp), pLd8 (47,015 bp), pLd9 (15,313 bp), pLd10 (39,563 bp), pLd11 (9833 bp) and pLd12 (3321 bp). Structural analysis of the repB promoters and the RepB proteins showed that eleven of the plasmids replicate via the theta-type mechanism, while only plasmid pLd3 replicates via a rolling-circle replication mechanism. Plasmids pLd2, pLd7 and pLd10 contain a highly similar operon involved in mobilisation of the plasmids. Examination of the twelve plasmids of L. lactis FM03P showed that 10 of the plasmids carry putative genes known to be important for growth and survival in the dairy environment. These genes encode technological functions such as lactose utilisation (lacR-lacABCDFEGX), citrate uptake (citQRP), peptide degradation (pepO and pepE) and oligopeptide uptake (oppDFBCA), uptake of magnesium and manganese (2 mntH, corA), exopolysaccharides production (eps operon), bacteriophage resistance (1 hsdM, 1 hsdR and 7 different hsdS genes of a type I restriction-modification system, an operon of three genes encoding a putative type II restriction-modification system and an abortive infection gene) and stress resistance (2 uspA, cspC and cadCA). Acquisition of these plasmids most likely facilitated the adaptation of the recipient strain to the dairy environment. Some plasmids were already lost during a single propagation step signifying their instability in the absence of a selective pressure.
conclusionsLactococcus lactis FM03P carries 12 plasmids important for its adaptation to the dairy environment. Some of the plasmids were easily lost demonstrating that propagation outside the dairy environment should be minimised when studying dairy isolates of L. lactis.
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