ArticleOptics express2018
Three-dimensional super-resolution high-throughput imaging by structured illumination STED microscopy.
Article in Optics express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 31 citations in OpenAlex.
- Evaluating the resolution of conventional optical microscopes through point spread function measurement.iScience · 2023Review
- Superresolution structured illumination microscopy reconstruction algorithms: a review.Light, science & applications · 2023Review
- Article
- Ultralow Laser Power Three-Dimensional Superresolution Microscopy Based on Digitally Enhanced STED.Biosensors · 2022Article
- Pupil function design for multifocal confocal, STED, and isoSTED microscopy.Applied optics · 2021Article
- Volumetric live cell imaging with three-dimensional parallelized RESOLFT microscopy.Nature biotechnology · 2021Article
- Nanoscale optical writing through upconversion resonance energy transfer.Science advances · 2021Article
- Sub-second, super-resolved imaging of biological systems using parallel EO-STED.Optics letters · 2020Article
- Strategies to maximize performance in STimulated Emission Depletion (STED) nanoscopy of biological specimens.Methods (San Diego, Calif.) · 2020Review
- Fluorescence imaging with tailored light.Nanophotonics (Berlin, Germany) · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Stimulated emission depletion (STED) microscopy is able to image fluorescence labeled samples with nanometer scale resolution. STED microscopy is typically a point-scanning method, limited by the high intensity requirement of the depletion beam. With the development of high peak power lasers, two dimensional parallel STED microscopy has been developed. Here, we develop the theoretical basis for extending STED microscopy to three dimensional imaging in parallel. This method uses structured illumination (SI) to generates a three dimensional depletion pattern. Compared to the two dimensional parallel STED microscopy, the 3D SI-STED microscopy generates intensity modulation along the light propagation direction without requiring higher laser power. This method not only achieves axial super-resolution of STED microscopy but also greatly reduces photobleaching and photodamage for 3D volumetric imaging.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.