Evidence map›Paper›PMID 30118659›Full record

ArticleTranslational research : the journal of laboratory and clinical medicine2018

Functional characterization of alternatively spliced GSN in head and neck squamous cell carcinoma.

Dylan Z Kelley, Emily L Flam, Theresa Guo, Ludmila V Danilova, Fernando T Zamuner, Craig Bohrson, Michael Considine, Eric J Windsor, Justin A Bishop, Chi Zhang and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational research : the journal of laboratory and clinical medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
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  4. Article
  5. Article
  6. Development of alternative splicing signature in lung squamous cell carcinoma.Medical oncology (Northwood, London, England) · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 2 institutions in 2 countries.

Dylan Z KelleyDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Emily L FlamDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Theresa GuoDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Ludmila V DanilovaDepartment of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland; Laboratory of Systems Biology and Computational Genetics, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
Fernando T ZamunerDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Craig BohrsonDepartment of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Michael ConsidineDepartment of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Eric J WindsorDepartment of Biotechnology, Maryland Holistics LLC, Ellicott City, Maryland.
Justin A BishopDepartment of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland.
Chi ZhangDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Wayne M KochDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland.
David SidranskyDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland.
William H WestraDepartment of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland.
Christine H ChungDepartment of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Joseph A CalifanoHead and Neck Cancer Center, Moores Cancer Center, University of California, San Diego, La Jolla, California; Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, La Jolla, California.
Sarah WheelanDepartment of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Alexander V FavorovDepartment of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland; Laboratory of Systems Biology and Computational Genetics, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
Liliana FloreaMcKusick-Nathans Institute of Genetic Medicine, Center for Computational Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Elana J FertigDepartment of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland; Department of Otolaryngology-Head and Neck Surgery (OHNS), University of California, San Francisco, California.
Daria A GaykalovaDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland. Electronic address: dgaykal@jhmi.edu.
Johns Hopkins University · USNational Foundation for Cancer Research · US

Funding

Translational Research Central ServicesP30CA006973 · NCI · JOHNS HOPKINS UNIVERSITY · PI WILLIAM George NELSON · 1985 to 2026
$208.6M
TUMOR SUPPRESOR GENE PROMOTER HYPERMETHYLATION FOR DETECTION OF HEAD AND NECKP50DE019032 · NIDCR · JOHNS HOPKINS UNIVERSITY · PI SIDRANSKY, DAVID · 2007 to 2016
$27.1M
Characterizing genome-wide alternative splicing in HPV related HNSCCR21DE025398 · NIDCR · JOHNS HOPKINS UNIVERSITY · PI GAYKALOVA, DARIA A · 2015 to 2016
$446k
NCI NIH HHS P30 CA006973NIDCR NIH HHS P50 DE019032NIDCR NIH HHS R21 DE025398
6 · The paper itself

Abstract

We have recently performed the characterization of alternative splicing events (ASEs) in head and neck squamous cell carcinoma, which allows dysregulation of protein expression common for cancer cells. Such analysis demonstrated a high ASE prevalence among tumor samples, including tumor-specific alternative splicing in the GSN gene.In vitro studies confirmed that overall expression of either ASE-GSN or wild-type GSN (WT-GSN) isoform inversely correlated with cell proliferation, whereas the high ratio of ASE-GSN to WT-GSN correlated with increased cellular invasion. Additionally, a change in expression of either isoform caused compensatory changes in expression of the other isoform. Our results suggest that the overall expression and the balance between GSN isoforms are mediating factors in proliferation, while increased overall expression of ASE-GSN is specific to cancer tissues. As a result, we propose ASE-GSN can serve not only as a biomarker of disease and disease progression, but also as a neoantigen for head and neck squamous cell carcinoma treatment, for which only a limited number of disease-specific targeted therapies currently exist.

Indexed as

Alternative SplicingCell Line, TumorCell MovementCell ProliferationCell SurvivalGelsolinGene Expression Regulation, NeoplasticHumansModels, BiologicalNeoplasm InvasivenessNeoplasm MetastasisSquamous Cell Carcinoma of Head and NeckGelsolin

Identifiers

PMID30118659
PMCPMC6218276
OpenAlexW2884923842

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.