ArticleMolecular ecology resources2019
Harnessing the MinION: An example of how to establish long-read sequencing in a laboratory using challenging plant tissue from Eucalyptus pauciflora.
Article in Molecular ecology resources, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Complete genome sequence ofMicrobiology resource announcements · 2026Article
- Cookbook for plant genome sequences.BMC genomics · 2026Review
- Long-read de novo assembly of the spekboom (Portulacaria afra) genome.Scientific data · 2025Article
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- A chromosome-level Mitragyna parvifolia genome unveils spirooxindole alkaloid diversification and mitraphylline biosynthesis.The Plant cell · 2025Article
- Pathways to Recovery: Genomics and Resistance Assays for Tree Species Devastated by the Myrtle Rust Pathogen.Molecular ecology · 2025Article
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- Duckweed genomes and epigenomes underlie triploid hybridization and clonal reproduction.Current biology : CB · 2025Article
- Chromosome-level haplotype-resolved genome assembly of bread wheat's wild relative Aegilops mutica.Scientific data · 2025Article
- Impact of DNA Extraction Methods on Gut Microbiome Profiles: A Comparative Metagenomic Study.Phenomics (Cham, Switzerland) · 2025Article
- AAVolve: Concatenated long-read deep sequencing enables whole capsid tracking during shuffled AAV library selection.Molecular therapy. Methods & clinical development · 2024Article
- Chromosome-Level Genome Assembly of the Australian Rainforest Tree Rhodamnia argentea (Malletwood).Genome biology and evolution · 2024Article
- A chromosome-scale reference genome of grasspea (Lathyrus sativus).Scientific data · 2024Article
- Article
- Two long read-based genome assembly and annotation of polyploidy woody plants, Hibiscus syriacus L. using PacBio and Nanopore platforms.Scientific data · 2023Article
- Draft genome assemblies of the avian louse Brueelia nebulosa and its associates using long-read sequencing from an individual specimen.G3 (Bethesda, Md.) · 2023Article
- An Efficient Method to Prepare Barcoded cDNA Libraries from Plant Callus for Long-Read Sequencing.Methods and protocols · 2023Article
- Whole genome sequencing in the palm of your hand: how to implement a MinION Galaxy-based workflow in a food safety laboratory for rapidFrontiers in microbiology · 2023Article
- Genomic Approaches for the Study of Flower Development in Floriculture Crops.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Establishing MinION Sequencing and Genome Assembly Procedures for the Analysis of the Rooibos (Plants (Basel, Switzerland) · 2022Article
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8 authors.
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Abstract
Long-read sequencing technologies are transforming our ability to assemble highly complex genomes. Realizing their full potential is critically reliant on extracting high-quality, high-molecular-weight (HMW) DNA from the organisms of interest. This is especially the case for the portable MinION sequencer which enables all laboratories to undertake their own genome sequencing projects, due to its low entry cost and minimal spatial footprint. One challenge of the MinION is that each group has to independently establish effective protocols for using the instrument, which can be time-consuming and costly. Here, we present a workflow and protocols that enabled us to establish MinION sequencing in our own laboratories, based on optimizing DNA extraction from a challenging plant tissue as a case study. Following the workflow illustrated, we were able to reliably and repeatedly obtain >6.5 Gb of long-read sequencing data with a mean read length of 13 kb and an N50 of 26 kb. Our protocols are open source and can be performed in any laboratory without special equipment. We also illustrate some more elaborate workflows which can increase mean and average read lengths if this is desired. We envision that our workflow for establishing MinION sequencing, including the illustration of potential pitfalls and suggestions of how to adapt it to other tissue types, will be useful to others who plan to establish long-read sequencing in their own laboratories.
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