ArticleGut microbes2019
Conventional culture methods with commercially available media unveil the presence of novel culturable bacteria.
Article in Gut microbes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed.
- Infant gut microbiota coverage by different culture media.BMC microbiology · 2026Article
- Characterization of goat rumen-derived isolates and their effects onFrontiers in veterinary science · 2026Article
- Degradation of Dioxins and DBF in Urban Soil Microcosms from Lausanne (Switzerland): Functional Performance of Indigenous Bacterial Strains.Microorganisms · 2025Article
- Electrochemical Immunodetection ofSensors (Basel, Switzerland) · 2025Article
- Effects of Short-Chain Fatty Acid Combinations Relevant to the Healthy and Dysbiotic Gut upon Candida albicans.Current microbiology · 2025Article
- Biosensing Strategies to Monitor Contaminants and Additives on Fish, Meat, Poultry, and Related Products.Biosensors · 2025Review
- Metagenome-guided culturomics for the targeted enrichment of gut microbes.Nature communications · 2025Article
- Bacterial profiling of colorectal cancer biopsies: a culture-based study in Indian patients.Frontiers in cellular and infection microbiology · 2025Article
- Evaluating agar-plating and dilution-to-extinction isolation methods for generating oak-associated microbial culture collections.ISME communications · 2025Article
- A vacuum lyophilization and bacterial tablet-based method for culture medium evaluation and potential use in probiotic products.Frontiers in microbiology · 2025Article
- Identification and Bioactivity Analysis of a NovelAntibiotics (Basel, Switzerland) · 2024Article
- Comparison of Taxonomic Resolutions of Various Typing Methods forIndian journal of microbiology · 2024Article
- Multi-omics analysis reveals the core microbiome and biomarker for nutrition degradation in alfalfa silage fermentation.mSystems · 2024Article
- Uncovering newMicrobiology spectrum · 2024Article
- Effects of Prebiotic Dietary Fibers on the Stimulation of the Mucin Secretion in Host Cells by In Vitro Gut Microbiome Consortia.Foods (Basel, Switzerland) · 2024Article
- A streamlined culturomics case study for the human gut microbiota research.Scientific reports · 2024Article
- The giant panda gut harbors a high diversity of lactic acid bacteria revealed by a novel culturomics pipeline.mSystems · 2024Article
- Culture-dependent identification of rare marine sediment bacteria from the Gulf of Mexico and Antarctica.bioRxiv : the preprint server for biology · 2024Article
- Advances in Culturomics Research on the Human Gut Microbiome: Optimizing Medium Composition and Culture Techniques for Enhanced Microbial Discovery.Journal of microbiology and biotechnology · 2024Review
- What Makes the Gut-Lung Axis Working? From the Perspective of Microbiota and Traditional Chinese Medicine.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent metagenomic analysis has revealed that our gut microbiota plays an important role in not only the maintenance of our health but also various diseases such as obesity, diabetes, inflammatory bowel disease, and allergy. However, most intestinal bacteria are considered 'unculturable' bacteria, and their functions remain unknown. Although culture-independent genomic approaches have enabled us to gain insight into their potential roles, culture-based approaches are still required to understand their characteristic features and phenotypes. To date, various culturing methods have been attempted to obtain these 'unculturable' bacteria, but most such methods require advanced techniques. Here, we have tried to isolate possible unculturable bacteria from a healthy Japanese individual by using commercially available media. A 16S rRNA (ribosomal RNA) gene metagenomic analysis revealed that each culture medium showed bacterial growth depending on its selective features and a possibility of the presence of novel bacterial species. Whole genome sequencing of these candidate strains suggested the isolation of 8 novel bacterial species classified in the Actinobacteria and Firmicutes phyla. Our approach indicates that a number of intestinal bacteria hitherto considered unculturable are potentially culturable and can be cultured on commercially available media. We have obtained novel gut bacteria from a healthy Japanese individual using a combination of comprehensive genomics and conventional culturing methods. We would expect that the discovery of such novel bacteria could illuminate pivotal roles for the gut microbiota in association with human health.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.