Evidence map›Paper›PMID 30116613›Full record

ArticleActa naturae

The Development and Study of Recombinant Immunoglobulin A to Hemagglutinins of the Influenza Virus.

T K Aliev, I G Dement'yeva, V A Toporova, V V Argentova, L P Pozdnyakova, M N Bokov, Yu A Votchitseva, D A Dolgikh, S D Varfolomeyev, P G Sveshnikov and 1 more

Abstract read
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In one paragraph

Article in Acta naturae. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

T K AlievLomonosov Moscow State University, Department of Chemistry, Leninskie gory 1, bldg. 3, Moscow, 119991, Russia.
I G Dement'yevaRussian Research Center for Molecular Diagnostics and Therapy, Simferopolsky Blvd. 8, Moscow, 117149 , Russia.
V A ToporovaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, Moscow, 117997, Russia.
V V ArgentovaLomonosov Moscow State University, Faculty of Biology, Leninskie gory 1, bldg. 12, Moscow, 119991 , Russia.
L P PozdnyakovaRussian Research Center for Molecular Diagnostics and Therapy, Simferopolsky Blvd. 8, Moscow, 117149 , Russia.
M N BokovRussian Research Center for Molecular Diagnostics and Therapy, Simferopolsky Blvd. 8, Moscow, 117149 , Russia.
Yu A VotchitsevaLomonosov Moscow State University, Faculty of Biology, Leninskie gory 1, bldg. 12, Moscow, 119991 , Russia.
D A DolgikhShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, Moscow, 117997, Russia.
S D VarfolomeyevLomonosov Moscow State University, Department of Chemistry, Leninskie gory 1, bldg. 3, Moscow, 119991, Russia.
P G SveshnikovRussian Research Center for Molecular Diagnostics and Therapy, Simferopolsky Blvd. 8, Moscow, 117149 , Russia.
M P KirpichnikovShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, Moscow, 117997, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We obtained recombinant variants of human antibody FI6 broadly specific to hemagglutinins of the influenza A virus. On the basis of a bi-promoter (CMV, hEF1-HTLV) vector, we developed genetic constructs for the expression of the heavy and light chains of the immunoglobulins of IgA1-, IgA2m1-, and IgG-isotypes. Following transfection and selection, stable Chinese hamster ovary (CHO) cell lines were produced. The antibodies of IgA1-, IgA2m1-, and IgG-isotypes were purified from culture media. We performed an immunochemical characterization and studied their interactions with influenza A strains of the H1N1- and H3N2-subtypes. It was shown that recombinant FI6 variants of the IgA-isotype retain the properties of the parental IgG antibody to demonstrate specificity to all the strains tested. The strongest binding was observed for the H1N1 subtype, which belongs to hemagglutinins of phylogenetic group I.

Indexed as

broadly neutralizing antibodiesIgA1IgA2m1immunoglobulin AInfluenza A virusrecombinant antibodies

Identifiers

PMID30116613
PMCPMC6087826

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.