ArticleThe ISME journal2019
Functional metagenomics reveals abundant polysaccharide-degrading gene clusters and cellobiose utilization pathways within gut microbiota of a wood-feeding higher termite.
Article in The ISME journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 151 citations in OpenAlex.
- Combining Yeast Display and Bacterial Genomic Library for the Unbiased Isolation of Novel Polysaccharide-Binding Peptides.International journal of molecular sciences · 2026Article
- Enzymatic Synergy-Driven Biotransformation Generates a Postbiotic-Rich Functional Matrix That Reprograms Gut Microbiota Metabolic Pathways Under Stress Conditions.International journal of molecular sciences · 2026Article
- Multi-omics analysis identifies gut microbiota-glutamine axis contributing to the pathogenesis of reflux esophagitis.Frontiers in microbiology · 2026Article
- Life inside a bag: multiomics insights into the bagworm species Eumeta crameri.DNA research : an international journal for rapid publication of reports on genes and genomes · 2025Article
- Termites and their gut microbiome in animal nutrition: Advances and biotechnological applications.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Review
- Article
- Genome-Scale Community Model-Guided Development of Bacterial Coculture for Lignocellulose Bioconversion.Biotechnology and bioengineering · 2025Article
- Review
- Article
- Effects of black soldier fly larvae on biotransformation and residues of spent mushroom substrate and wet distiller's grains.Scientific reports · 2024Article
- Metagenomic functional profiling: to sketch or not to sketch?Bioinformatics (Oxford, England) · 2024Article
- Comparative genomic analysis of Planctomycetota potential for polysaccharide degradation identifies biotechnologically relevant microbes.BMC genomics · 2024Article
- Elucidating the structure, and composition of bacterial symbionts in the gut regions of wood-feeding termite,Frontiers in microbiology · 2024Article
- Metagenomics analysis of bacterial community structure from wood- and soil-feeding termites: metabolic pathways and functional structures toward the degradation of lignocellulose and recalcitrant compounds.Frontiers in microbiology · 2024Article
- Exploring the microbial diversity and characterization of cellulase and hemicellulase genes in goat rumen: a metagenomic approach.BMC biotechnology · 2023Article
- Discovery of novel alkaline-tolerant xylanases from fecal microbiota of dairy cows.Biotechnology for biofuels and bioproducts · 2023Article
- Different roles of host and habitat in determining the microbial communities of plant-feeding true bugs.Microbiome · 2023Article
- Predation of oomycetes by myxobacteria via a specialized CAZyme system arising from adaptive evolution.The ISME journal · 2023Article
- Species-Level Taxonomic Characterization of Uncultured Core Gut Microbiota of Plateau Pika.Microbiology spectrum · 2023Article
- Molecular insights into the evolution of woody plant decay in the gut of termites.Science advances · 2023Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
Abstract
Plant cell-wall polysaccharides constitute the most abundant but recalcitrant organic carbon source in nature. Microbes residing in the digestive tract of herbivorous bilaterians are particularly efficient at depolymerizing polysaccharides into fermentable sugars and play a significant support role towards their host's lifestyle. Here, we combine large-scale functional screening of fosmid libraries, shotgun sequencing, and biochemical assays to interrogate the gut microbiota of the wood-feeding "higher" termite Globitermes brachycerastes. A number of putative polysaccharide utilization gene clusters were identified with multiple fibrolytic genes. Our large-scale functional screening of 50,000 fosmid clones resulted in 464 clones demonstrating plant polysaccharide-degrading activities, including 267 endoglucanase-, 24 exoglucanase-, 72 β-glucosidase-, and 101 endoxylanase-positive clones. We sequenced 173 functionally active clones and identified ~219 genes encoding putative carbohydrate-active enzymes (CAZymes) targeting cellulose, hemicellulose and pectin. Further analyses revealed that 68 of 154 contigs encode one or more CAZyme, which includes 35 examples of putative saccharolytic operons, suggesting that clustering of CAZymes is common in termite gut microbial inhabitants. Biochemical characterization of a representative xylanase cluster demonstrated that constituent enzymes exhibited complementary physicochemical properties and saccharolytic capabilities. Furthermore, diverse cellobiose-metabolizing enzymes include β-glucosidases, cellobiose phosphorylases, and phopho-6-β-glucosidases were identified and functionally verified, indicating that the termite gut micro-ecosystem utilizes diverse metabolic pathways to interconnect hydrolysis and central metabolism. Collectively, these results provide an in-depth view of the adaptation and digestive strategies employed by gut microbiota within this tiny-yet-efficient host-associated ecosystem.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.