ArticleDevelopment (Cambridge, England)2018
Geometrical confinement controls the asymmetric patterning of brachyury in cultures of pluripotent cells.
Article in Development (Cambridge, England), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Size-Modulated Mesoderm-Endoderm Divergence and Myocardial Cavitation in Micropatterned Cardioids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- From genes to germ layers: virtual twins of gastruloids.NPJ systems biology and applications · 2026Review
- FATE-MAP predicts teratogenicity and human gastrulation failure modes by integrating deep learning and mechanistic modeling.Nature communications · 2026Article
- A bioengineering approach to guide non-integrated stem cell-derived embryo model towards yolk sac development and hematopoiesis.Frontiers in cell and developmental biology · 2026Article
- Size-Modulated Mesoderm-Endoderm Divergence and Myocardial Cavitation in Micropatterned Cardioids.bioRxiv : the preprint server for biology · 2025Article
- Poly(norepinephrine)-Mediated Universal Surface Modification for Patterning Human Pluripotent Stem Cell Culture and Differentiation.ACS biomaterials science & engineering · 2024Article
- In Vitro Models of Cardiovascular Disease: Embryoid Bodies, Organoids and Everything in Between.Biomedicines · 2024Review
- The logic of monsters: development and morphological diversity in stem-cell-based embryo models.Interface focus · 2024Review
- An explainable map of human gastruloid morphospace reveals gastrulation failure modes and predicts teratogens.bioRxiv : the preprint server for biology · 2024Article
- ARTseq-FISH reveals position-dependent differences in gene expression of micropatterned mESCs.Nature communications · 2024Article
- Optogenetic control of Wnt signaling models cell-intrinsic embryogenic patterning using 2D human pluripotent stem cell culture.Development (Cambridge, England) · 2023Article
- Symmetry Breaking of Human Pluripotent Stem Cells (hPSCs) in Micropattern Generates a Polarized Spinal Cord-Like Organoid (pSCO) with Dorsoventral Organization.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Spinal Cord Organoids to Study Motor Neuron Development and Disease.Life (Basel, Switzerland) · 2023Review
- Gradient to sectioning CUBE workflow for the generation and imaging of organoids with localized differentiation.Communications biology · 2023Article
- Defined Microenvironments Trigger In Vitro Gastrulation in Human Pluripotent Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- SyNPL: Synthetic Notch pluripotent cell lines to monitor and manipulate cell interactions in vitro and in vivo.Development (Cambridge, England) · 2022Article
- Cell-state transitions and collective cell movement generate an endoderm-like region in gastruloids.eLife · 2022Article
- Cell position within human pluripotent stem cell colonies determines apical specialization via an actin cytoskeleton-based mechanism.Stem cell reports · 2022Article
- Sculpting with stem cells: how models of embryo development take shape.Development (Cambridge, England) · 2021Article
- Quantitative developmental biology in vitro using micropatterning.Development (Cambridge, England) · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Diffusible signals are known to orchestrate patterning during embryogenesis, yet diffusion is sensitive to noise. The fact that embryogenesis is remarkably robust suggests that additional layers of regulation reinforce patterning. Here, we demonstrate that geometrical confinement orchestrates the spatial organisation of initially randomly positioned subpopulations of spontaneously differentiating mouse embryonic stem cells. We use micropatterning in combination with pharmacological manipulations and quantitative imaging to dissociate the multiple effects of geometry. We show that the positioning of a pre-streak-like population marked by brachyury (T) is decoupled from the size of its population, and that breaking radial symmetry of patterns imposes polarised patterning. We provide evidence for a model in which the overall level of diffusible signals together with the history of the cell culture define the number of T
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.