Evidence map›Paper›PMID 30115120›Full record

ArticleArthritis research & therapy2018

Aberrantly expressed messenger RNAs and long noncoding RNAs in degenerative nucleus pulposus cells co-cultured with adipose-derived mesenchymal stem cells.

Zhihua Han, Jiandong Wang, Liang Gao, Qiugen Wang, Jianhong Wu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arthritis research & therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 18 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 2 countries.

Zhihua HanOrthopedic Traumatology, Shanghai Trauma and Emergency Center, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, Xin Songjiang Road 650, Shanghai, 201620, China.
Jiandong WangOrthopedic Traumatology, Shanghai Trauma and Emergency Center, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, Xin Songjiang Road 650, Shanghai, 201620, China.
Liang GaoCenter of Experimental Orthopaedics and Department of Orthopaedic Surgery, Saarland University Medical Center, Homburg/Saar, Germany.
Qiugen WangOrthopedic Traumatology, Shanghai Trauma and Emergency Center, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, Xin Songjiang Road 650, Shanghai, 201620, China.
Jianhong WuOrthopedic Traumatology, Shanghai Trauma and Emergency Center, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, Xin Songjiang Road 650, Shanghai, 201620, China. wujianhong1978@hotmail.com.
Shanghai First People's Hospital · CNGoethe University Frankfurt · DESaarland University · DEXinHua Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundStem cell therapy is considered as a promising alternative to treat intervertebral disc degeneration (IDD). Extensive work had been done on identifying and comparing different types of candidate stem cells, both in vivo and in vitro. However, few studies have shed light on degenerative nucleus pulposus cells (NPCs), especially their biological behavior under the influence of exogenous stem cells, specifically the gene expression and regulation pattern. In the present study, we aimed to determine messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs), which are differentially expressed during the co-culturing process with adipose-derived mesenchymal stem cells (ASCs) and to explore the involved signaling pathways and the regulatory networks.

methodsWe compared degenerative NPCs co-cultured with ASCs with those cultured solely using lncRNA-mRNA microarray analysis. Based on these data, we investigated the significantly regulated signaling pathways based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database. Moreover, 23 micro RNAs (miRNAs), which were demonstrated to be involved in IDD were chosen; we investigated their theoretic regulatory importance associated with our microarray data.

resultsWe found 632 lncRNAs and 1682 mRNAs were differentially expressed out of a total of 40,716 probes. We then confirmed the microarray data by real-time PCR. Furthermore, we demonstrated 197 upregulated, and 373 downregulated Gene Ontology terms and 176 significantly enriched pathways, such as the mitogen-activated protein kinase (MAPK) pathway. Also, a signal-net was constructed to reveal the interplay among differentially expressed genes. Meanwhile, a mRNA-lncRNA co-expression network was constructed for the significantly changed mRNAs and lncRNAs. Also, the competing endogenous RNA (ceRNA) network was built.

conclusionOur results present the first comprehensive identification of differentially expressed lncRNAs and mRNAs of degenerative NPCs, altered by co-culturing with ASCs, and outline the gene expression regulation pattern. These may provide valuable information for better understanding of stem cell therapy and potential candidate biomarkers for IDD treatment.

Indexed as

Cells, CulturedCoculture TechniquesHumansIntervertebral Disc DegenerationMesenchymal Stem CellsNucleus PulposusRNA, Long NoncodingRNA, MessengerTranscriptomeRNA, Long NoncodingRNA, MessengerAdipose-derived mesenchymal stem cellsCo-cultureGene microarray analysisIntervertebral disc degenerationLong non-coding RNAsMicro RNAsNucleus pulposus cells

Identifiers

PMID30115120
PMCPMC6097446
OpenAlexW2887768884

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.