ArticleJournal of the American Chemical Society2018
Measuring Ligand Binding Kinetics to Membrane Proteins Using Virion Nano-oscillators.
Article in Journal of the American Chemical Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Real-Time Binding Kinetics of Membrane Protein-Protein Interactions in a Membraneless Setting.Analytical chemistry · 2025Article
- Plasmonic DNA-Barcoded Virion Nano-Oscillators for Multiplexed Quantification of Small-Molecule Binding Kinetics to Membrane Proteins.Angewandte Chemie (International ed. in English) · 2025Article
- Precise Sizing and Collision Detection of Functional Nanoparticles by Deep Learning Empowered Plasmonic Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Advances in surface plasmon resonance for analyzing active components in traditional Chinese medicine.Journal of pharmaceutical analysis · 2024Review
- Rapid Degradation of the Human ACE2 Receptor Upon Binding and Internalization of SARS-Cov-2-Spike-RBD Protein.bioRxiv : the preprint server for biology · 2024Article
- Surface Plasmon Resonance Biosensors: A Review of Molecular Imaging with High Spatial Resolution.Biosensors · 2024Review
- Review
- Expression and fusogenic activity of SARS CoV-2 Spike protein displayed in the HSV-1 Virion.bioRxiv : the preprint server for biology · 2023Article
- Plasmonic Scattering Microscopy for Label-Free Imaging of Molecular Binding Kinetics: From Single Molecules to Single Cells.Chemistry methods : new approaches to solving problems in chemistry · 2023Article
- Ligand-binding assay based on microfluidic chemotaxis of porphyrin receptors.Chemical science · 2022Article
- Label-Free Multimetric Measurement of Molecular Binding Kinetics by Electrical Modulation of a Flexible Nanobiolayer.ACS sensors · 2022Article
- Quantification of Single-Molecule Protein Binding Kinetics in Complex Media with Prism-Coupled Plasmonic Scattering Imaging.ACS sensors · 2021Article
- Charge-Sensitive Optical Detection of Small Molecule Binding Kinetics in Normal Ionic Strength Buffer.ACS sensors · 2021Article
- Future perspective: high-throughput construction of new ultrasensitive cytokine and virion liquid chips for high-throughput screening (HTS) of anti-inflammatory drugs or clinical diagnosis and treatment of inflammatory diseases.Analytical and bioanalytical chemistry · 2020Article
- Virion Display: A High-Throughput Method to Express Functional Membrane Proteins.Current protocols in molecular biology · 2020Article
- Developments and Applications of Functional Protein Microarrays.Molecular & cellular proteomics : MCP · 2020Review
- Roles of entropic and solvent damping forces in the dynamics of polymer tethered nanoparticles and implications for single molecule sensing.Chemical science · 2020Article
- Quantifying Ligand-Protein Binding Kinetics with Self-Assembled Nano-oscillators.Analytical chemistry · 2019Article
- Development and application of a high-content virion display human GPCR array.Nature communications · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Membrane proteins play vital roles in cellular signaling processes and serve as the most popular drug targets. A key task in studying cellular functions and developing drugs is to measure the binding kinetics of ligands with the membrane proteins. However, this has been a long-standing challenge because one must perform the measurement in a membrane environment to maintain the conformations and functions of the membrane proteins. Here, we report a new method to measure ligand binding kinetics to membrane proteins using self-assembled virion oscillators. Virions of human herpesvirus were used to display human G-protein-coupled receptors (GPCRs) on their viral envelopes. Each virion was then attached to a gold-coated glass surface via a flexible polymer to form an oscillator and driven into oscillation with an alternating electric field. By tracking changes in the oscillation amplitude in real-time with subnanometer precision, the binding kinetics between ligands and GPCRs was measured. We anticipate that this new label-free detection technology can be readily applied to measure small or large ligand binding to any type of membrane proteins and thus contribute to the understanding of cellular functions and screening of drugs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.