ArticleCurrent protocols in protein science2018
In Situ Imaging of Tryptic Peptides by MALDI Imaging Mass Spectrometry Using Fresh-Frozen or Formalin-Fixed, Paraffin-Embedded Tissue.
Article in Current protocols in protein science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- The evolving landscape of spatial proteomics technologies in the AI age.Fundamental research · 2026Review
- Integrative Spatial Omics for Systems-Level Mapping of Pathological Niches.bioRxiv : the preprint server for biology · 2025Article
- Spatial Proteomics by Parallel Accumulation-Serial Fragmentation Supported MALDI MS/MS Imaging: A First Glance Into Multiplexed and Spatial Peptide Identification.Rapid communications in mass spectrometry : RCM · 2025Article
- 6-Aza-2-Thiothymine as an Alternative Matrix for Spatial Proteomics with MALDI-MSI.International journal of molecular sciences · 2024Article
- MALDI MSI Protocol for Spatial Bottom-Up Proteomics at Single-Cell Resolution.Journal of proteome research · 2024Article
- Mass Spectrometry Imaging with Trapped Ion Mobility Spectrometry Enables Spatially Resolved Chondroitin, Dermatan, and Hyaluronan Glycosaminoglycan Oligosaccharide AnalysisAnalytical chemistry · 2024Article
- Thermal Denaturation of Fresh Frozen Tissue Enhances Mass Spectrometry Detection of Peptides.Analytical chemistry · 2024Article
- Review
- MALDI-IHC-Guided In-Depth Spatial Proteomics: Targeted and Untargeted MSI Combined.Analytical chemistry · 2023Article
- Device-Controlled Microcondensation for Spatially Confined On-Tissue Digests in MALDI Imaging ofPharmaceuticals (Basel, Switzerland) · 2022Article
- An optimized MALDI MSI protocol for spatial detection of tryptic peptides in fresh frozen prostate tissue.Proteomics · 2022Article
- Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry Imaging of L-4-Phenylalanineboronic Acid (BPA) in a Brain Tumor Model Rat for Boron Neutron Capture Therapy (BNCT).Mass spectrometry (Tokyo, Japan) · 2022Article
- Metabolic Links to Socioeconomic Stresses Uniquely Affecting Ancestry in Normal Breast Tissue at Risk for Breast Cancer.Frontiers in oncology · 2022Article
- A Mass Spectrometry Imaging Based Approach for Prognosis Prediction in UICC Stage I/II Colon Cancer.Cancers · 2021Article
- Removal of optimal cutting temperature (O.C.T.) compound from embedded tissue for MALDI imaging of lipids.Analytical and bioanalytical chemistry · 2021Article
- Neurodegenerative Proteinopathies in the Proteoform Spectrum-Tools and Challenges.International journal of molecular sciences · 2021Review
- MALDI-MSI spatially maps N-glycan alterations to histologically distinct pulmonary pathologies following irradiation.Scientific reports · 2020Article
- Mapping and Identification of Native Proteins of Developing Teeth in Mouse Mandibles.Analytical chemistry · 2020Article
- Correlation of molecular and morphologic effects of thermoembolization in a swine model using mass spectrometry imaging.Journal of mass spectrometry : JMS · 2020Article
- A recommended and verified procedure for in situ tryptic digestion of formalin-fixed paraffin-embedded tissues for analysis by matrix-assisted laser desorption/ionization imaging mass spectrometry.Journal of mass spectrometry : JMS · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Tryptic peptide imaging is a primary workflow for matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) and has led to new information reporting highly multiplexed protein localization. Technological advances within the last few years have produced robust tools for automated spraying of both matrix and enzymes. When combined with high-mass-resolution and high-mass-accuracy instrumentation, studies now generally result in two-dimensional mapping of well over 1,000 peptide peaks. This protocol describes sample preparation, spraying, and application of enzymes and matrices, and MALDI FT-ICR instrumental considerations for two-dimensional mapping of tryptic peptides from fresh-frozen or formalin-fixed, paraffin-embedded tissue sections. Procedures for extraction of tryptic peptides from tissue sections for LC-MS/MS identification are also described. © 2018 by John Wiley & Sons, Inc.
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