Evidence map›Paper›PMID 30114278›Full record

ArticlePloS one2018

PPARγ is reduced in the airways of non-CF bronchiectasis subjects and is inversely correlated with the presence of Pseudomonas aeruginosa.

Lucy D Burr, Geraint B Rogers, Alice C-H Chen, Steven L Taylor, Simon D Bowler, Rebecca L Keating, Megan L Martin, Sumaira Z Hasnain, Michael A McGuckin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Unleashing AdipoRon's Potential: A Fresh Approach to TackleJournal of inflammation research · 2024
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Lucy D BurrImmunity, Infection and Inflammation Program, Mater Research-University of Queensland, Translational Research Institute, Wooloongabba, QLD, Australia.ORCID 0000-0003-4010-3787
Geraint B RogersSAHMRI Infection and Immunity Theme, School of Medicine, Flinders University, Adelaide, Australia.
Alice C-H ChenImmunity, Infection and Inflammation Program, Mater Research-University of Queensland, Translational Research Institute, Wooloongabba, QLD, Australia.
Steven L TaylorSAHMRI Infection and Immunity Theme, School of Medicine, Flinders University, Adelaide, Australia.
Simon D BowlerDepartment of Respiratory Medicine, Mater Misericordiae Brisbane Ltd, South Brisbane, QLD, Australia.
Rebecca L KeatingDepartment of Respiratory Medicine, Mater Misericordiae Brisbane Ltd, South Brisbane, QLD, Australia.
Megan L MartinDepartment of Respiratory Medicine, Mater Misericordiae Brisbane Ltd, South Brisbane, QLD, Australia.
Sumaira Z HasnainImmunity, Infection and Inflammation Program, Mater Research-University of Queensland, Translational Research Institute, Wooloongabba, QLD, Australia.
Michael A McGuckinImmunity, Infection and Inflammation Program, Mater Research-University of Queensland, Translational Research Institute, Wooloongabba, QLD, Australia.
Translational Research Institute · AUMater Misericordiae Hospital · AUFlinders University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundChronic airway inflammation in conditions such as cystic fibrosis (CF) and non-CF bronchiectasis is characterised by a predominant neutrophilic inflammatory response, commonly due to the presence of pathogenic bacteria such as Pseudomonas aeruginosa. We hypothesised that down-regulation of the anti-inflammatory nuclear transcription regulator peroxisome proliferator-activated receptor gamma (PPARγ in non-CF bronchiectasis subjects may explain why this exuberant neutrophilic inflammation is able to persist unchecked in the inflamed airway.

methodsPPARγ gene expression was assessed in bronchoalveolar lavage fluid (BAL) of 35 macrolide naïve non-CF bronchiectasis subjects and compared with that in 20 healthy controls. Human RNA was extracted from pelleted BAL and PPARγ expression was determined by reverse-transcription quantitative PCR. Bacterial DNA was extracted from paired induced sputum and total bacterial load was determined by 16S rRNA qPCR. Quantification of individual bacterial species was achieved by qPCR.

resultsPPARγ expression was lower in subjects with non-CF bronchiectasis compared with healthy control subjects (control: 1.00, IQR 0.55-1.44, n = 20 vs. Bronchiectasis: 0.49, IQR 0.12-0.89; n = 35; p<0.001, Mann-Whitney U test). This lower PPARγ expression correlated negatively with Pseudomonas aeruginosa (r = -0.53, n = 31; p = 0.002). No significant association was seen between PPARγ and total bacterial levels or levels Haemophilus influenzae.

conclusionPPARγ is expressed in low levels in the airways of non-CF bronchiectasis subjects, despite an aggressive inflammatory response. This low level PPARγ expression is particularly associated with the presence of high levels of P. aeruginosa, and may represent an intrinsic link with this bacterial pathogen.

Indexed as

Pseudomonas aeruginosaAdultBacterial LoadBronchiectasisBronchoalveolar Lavage FluidFemaleGene Expression RegulationHumansMaleMiddle AgedPPAR gammaPPAR gamma

Identifiers

PMID30114278
PMCPMC6095532
OpenAlexW2886146796

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.