ArticleACS applied materials & interfaces2018
Duplex-Specific Nuclease-Amplified Detection of MicroRNA Using Compact Quantum Dot-DNA Conjugates.
Article in ACS applied materials & interfaces, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 69 citations in OpenAlex.
- Recent advances in nanozyme assisted miRNA biosensing for disease diagnosis.Mikrochimica acta · 2026Review
- Diagnosis of Endometriosis: Dual-Amplification Strategy Driven by Copper Nanoclusters.Analytical chemistry · 2026Article
- Applicability of Quantum Dots in Breast Cancer Diagnostic and Therapeutic Modalities-A State-of-the-Art Review.Nanomaterials (Basel, Switzerland) · 2024Review
- MiR-25802: a potential target for treating Alzheimer's disease by regulating neuroinflammation.Frontiers in immunology · 2024Article
- State-of-the-Art Fluorescent Probes: Duplex-Specific Nuclease-Based Strategies for Early Disease Diagnostics.Biosensors · 2022Review
- Formation of miRNA Nanoprobes-Conjugation Approaches Leading to the Functionalization.Molecules (Basel, Switzerland) · 2022Review
- A Rapid, Fluorescence Switch-On Biosensor for Early Diagnosis of Sorghum Mosaic Virus.Biosensors · 2022Article
- DNA-gold nanoprobe-based integrated biosensing technology for non-invasive liquid biopsy of serum miRNA: A new frontier in prostate cancer diagnosis.Nanomedicine : nanotechnology, biology, and medicine · 2022Article
- Role of Nano-miRNAs in Diagnostics and Therapeutics.International journal of molecular sciences · 2022Review
- Quantum Dots for Cancer-Related miRNA Monitoring.ACS sensors · 2022Review
- The advances in nanomedicine for bone and cartilage repair.Journal of nanobiotechnology · 2022Review
- Noble Metal Nanoparticle Biosensors: From Fundamental Studies toward Point-of-Care Diagnostics.Accounts of chemical research · 2022Review
- Two-step formulation of magnetic nanoprobes for microRNA capture.RSC advances · 2022Article
- Microscopies Enabled by Photonic Metamaterials.Sensors (Basel, Switzerland) · 2022Review
- A novel DSN-based fluorescence assay for MicroRNA-133a detection and its application for LVH diagnosis in maintenance hemodialysis patients.Journal of clinical laboratory analysis · 2020Article
- Transcriptomics in Alzheimer's Disease: Aspects and Challenges.International journal of molecular sciences · 2020Review
- Using gold nanoparticles to detect single-nucleotide polymorphisms: toward liquid biopsy.Beilstein journal of nanotechnology · 2020Review
- Multiplexed fluorometric determination for three microRNAs in acute myocardial infarction by using duplex-specific nuclease and MoSMikrochimica acta · 2019Article
- Signal amplification method for miR-205 assay through combining graphene oxide with duplex-specific nuclease.RSC advances · 2019Article
- Rolling Circle Transcription-Amplified Hierarchically Structured Organic-Inorganic Hybrid RNA Flowers for Enzyme Immobilization.ACS applied materials & interfaces · 2019Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Advances in nanotechnology have provided new opportunities for the design of next-generation nucleic acid biosensors and diagnostics. Indeed, combining advances in functional nanoparticles, DNA nanotechnology, and nuclease-enzyme-based amplification can give rise to new assays with advantageous properties. In this work, we developed a microRNA (miRNA) assay using bright fluorescent quantum dots (QDs), simple DNA probes, and the enzyme duplex-specific nuclease. We employed an isothermal target-recycling mechanism, where a single miRNA target triggers the cleavage of many DNA signal probes. The incorporation of DNA-functionalized QDs enabled a quantitative fluorescent readout, mediated by Förster resonance energy transfer (FRET)-based interaction with the DNA signal probes. Our approach splits the reaction in two, performing the enzyme-mediated amplification and QD-based detection steps separately such that each reaction could be optimized for performance of the active components. Target recycling gave ca. 3 orders of magnitude amplification, yielding highly sensitive detection with a limit of 42 fM (or 1.2 amol) of miR-148, with excellent selectivity versus mismatched sequences and other miRNAs. Furthermore, we used an alternative target (miR-21) and FRET pair for direct and absolute quantification of miR-21 in RNA extracts from human cancer and normal cell lines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.