ArticleLab on a chip2018
Automated microfluidic droplet sampling with integrated, mix-and-read immunoassays to resolve endocrine tissue secretion dynamics.
Article in Lab on a chip, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Label-Free Optical Sensor for Real-Time Monitoring of Insulin Secretion from Single Human Pancreatic Islets.Sensors (Basel, Switzerland) · 2026Article
- Dynamic sampling fromLab on a chip · 2024Article
- Unveiling islet heterogeneity using an automated microfluidic imaging system.Scientific reports · 2024Article
- Development of a mix-and-read assay for human asprosin using antibody-oligonucleotide probes and thermofluorimetric analysis.Analytical methods : advancing methods and applications · 2024Article
- Microfluidic 3D hepatic cultures integrated with a droplet-based bioanalysis unit.Biosensors & bioelectronics · 2024Article
- Droplet-based μChopper device with a 3D-printed pneumatic valving layer and a simple photometer for absorbance based fructosamine quantification in human serum.The Analyst · 2023Article
- Monitoring hormone and small molecule secretion dynamics from islets-on-chip.Analytical and bioanalytical chemistry · 2023Article
- Pancreatic islet organoids-on-a-chip: how far have we gone?Journal of nanobiotechnology · 2022Review
- Increasing insulin measurement throughput by fluorescence anisotropy imaging immunoassays.Analytica chimica acta · 2022Article
- Autologous Human Immunocompetent White Adipose Tissue-on-Chip.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- A low-cost self-dispersing method of droplet array generation enabled by a simple reusable mask for bioanalysis and bioassays.Analytical and bioanalytical chemistry · 2022Article
- Engineering islets from stem cells for advanced therapies of diabetes.Nature reviews. Drug discovery · 2021Review
- A microfluidic system for monitoring glucagon secretion from human pancreatic islets of Langerhans.Analytical methods : advancing methods and applications · 2021Article
- Active Flow Control and Dynamic Analysis in Droplet Microfluidics.Annual review of analytical chemistry (Palo Alto, Calif.) · 2021Review
- The Inflammatory Profile of Obesity and the Role on Pulmonary Bacterial and Viral Infections.International journal of molecular sciences · 2021Review
- Building Biomimetic Potency Tests for Islet Transplantation.Diabetes · 2021Review
- Nucleic-Acid Driven Cooperative Bioassays Using Probe Proximity or Split-Probe Techniques.Analytical chemistry · 2021Review
- Article
- Programmable µChopper Device with On-Chip Droplet Mergers for Continuous Assay Calibration.Micromachines · 2020Article
- Profiling Glucose-Stimulated and M3 Receptor-Activated Insulin Secretion Dynamics from Islets of Langerhans Using an Extended-Lifetime Fluorescence Dye.Analytical chemistry · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A fully automated droplet generation and analysis device based on pressure driven push-up valves for precise pumping of fluid and volumetric metering has been developed for high resolution hormone secretion sampling and measurement. The device consists of a 3D-printer templated reservoir for single cells or single tissue culturing, a Y-shaped channel for reagents and sample mixing, a T-junction channel for droplet formation, a reference channel to overcome drifts in fluorescence signal, and a long droplet storage channel allowing incubation for homogeneous immunoassays. The droplets were made by alternating peristaltic pumping of aqueous and oil phases. Device operation was automated, giving precise control over several droplet parameters such as size, oil spacing, and ratio of sample and reference droplets. By integrating an antibody-oligonucleotide based homogeneous immunoassay on-chip, high resolution temporal sampling into droplets was combined with separation-free quantification of insulin secretion from single islets of Langerhans using direct optical readout from the droplets. Quantitative assays of glucose-stimulated insulin secretion were demonstrated at 15 second temporal resolution while detecting as low as 10 amol per droplet, revealing fast insulin oscillations that mirror well-known intracellular calcium signals. This droplet sampling and direct optical analysis approach effectively digitizes the secretory time record from cells into droplets, and the system should be generalizable to a variety of cells and tissue types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.