ArticleScientific reports2018
Multiplex mutagenesis of four clustered CrRLK1L with CRISPR/Cas9 exposes their growth regulatory roles in response to metal ions.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 57 citations in OpenAlex.
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- Differential expression and localization of expansins inFrontiers in plant science · 2025Article
- Genome-wide analysis of the CrRLK1L gene family inFrontiers in plant science · 2025Article
- Genome-Wide Identification and Analysis ofPlants (Basel, Switzerland) · 2023Article
- Multiplex Editing of theThe CRISPR journal · 2023Article
- Lateral Root Initiation in Cucumber (International journal of molecular sciences · 2023Article
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- Genome-Wide Re-Identification and Analysis of CrRLK1Ls in Tomato.International journal of molecular sciences · 2023Article
- Advances in Receptor-like Protein Kinases in Balancing Plant Growth and Stress Responses.Plants (Basel, Switzerland) · 2023Review
- Characterization of Transcriptome Dynamics during Early Fruit Development in Olive (International journal of molecular sciences · 2023Article
- CRISPR/Cas tool designs for multiplex genome editing and its applications in developing biotic and abiotic stress-resistant crop plants.Molecular biology reports · 2022Review
- Genetic and physiological characterization of sunflower resistance provided by the wild-derived OrTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Systematic Analysis of Tobacco CrRLK1L Family Genes and Functional Identification ofFrontiers in plant science · 2022Article
- Malectin/Malectin-like domain-containing proteins: A repertoire of cell surface molecules with broad functional potential.Cell surface (Amsterdam, Netherlands) · 2021Review
- Genome-wide identification and analysis of Catharanthus roseus RLK1-like kinases in Nicotiana benthamiana.BMC plant biology · 2021Article
- Induced Genetic Variations in Fruit Trees Using New Breeding Tools: Food Security and Climate Resilience.Plants (Basel, Switzerland) · 2021Review
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Authors and funding
9 authors at 3 institutions in 1 country.
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Abstract
Resolving functions of closely linked genes is challenging or nearly impossible with classical genetic tools. Four members of the Catharanthus roseus receptor-like kinase 1-like (CrRLK1L) family are clustered on Arabidopsis chromosome five. To resolve the potentially redundant functions of this subclass of CrRLK1Ls named MEDOS1 to 4 (MDS1 to 4), we generated a single CRISPR/Cas9 transformation vector using a Golden Gate based cloning system to target all four genes simultaneously. We introduce single mutations within and deletions between MDS genes as well as knock-outs of the whole 11 kb gene cluster. The large MDS cluster deletion was inherited in up to 25% of plants lacking the CRISPR/Cas9 construct in the T2 generation. In contrast to described phenotypes of already characterized CrRLK1L mutants, quadruple mds knock-outs were fully fertile, developed normal root hairs and trichomes and responded to pharmacological inhibition of cellulose biosynthesis similar to wildtype. Recently, we demonstrated the role of four CrRLK1L in growth adaptation to metal ion stress. Here we show the involvement of MDS genes in response to Ni
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