ArticlePloS one2018
Cellular reagents for diagnostics and synthetic biology.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Rapid Point-of-Care Detection ofAnimals : an open access journal from MDPI · 2026Article
- Engineering and Assay of Enzymes for Expanded Genetic Alphabet Replication.Biochemistry · 2025Article
- A lyophilized open-source RT-LAMP assay for molecular diagnostics in resource-limited settings.Life science alliance · 2025Article
- Exploring the Correlation between Selected Oral Commensals and Deoxyribonucleic Acid Degradation in Pulp Tissue Extracted from Primary Teeth Stored in Dry and Wet Conditions: AnInternational journal of clinical pediatric dentistry · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Smartphone-readable RPA-LFA for the high-sensitivity detection of Leishmania kDNA using nanophosphor reporters.PLoS neglected tropical diseases · 2023Article
- Kinetics of elementary steps in loop-mediated isothermal amplification (LAMP) show that strand invasion during initiation is rate-limiting.Nucleic acids research · 2023Article
- Preparation and Use of Cellular Reagents: A Low-resource Molecular Biology Reagent Platform.Current protocols · 2022Article
- A Rapid, Highly Sensitive and Open-Access SARS-CoV-2 Detection Assay for Laboratory and Home Testing.Frontiers in molecular biosciences · 2022Article
- Unlocking SARS-CoV-2 detection in low- and middle-income countries.Cell reports methods · 2021Article
- News Feature: Biology research, no cells required: By borrowing the machinery of life from broken cells, researchers are producing novel proteins, cheap lab reagents, and just-add-water vaccines.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Lyophilization of premixed COVID-19 diagnostic RT-qPCR reactions enables stable long-term storage at elevated temperature.Biotechnology journal · 2021Article
- Producing molecular biology reagents without purification.PloS one · 2021Article
- Optimization of Loop-Mediated Isothermal Amplification (LAMP) reaction mixture for biosensor applications.MethodsX · 2021Article
- iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2.Virus research · 2020Article
- DNA amplification with in situ nucleoside to dNTP synthesis, using a single recombinant cell lysate of E. coli.Scientific reports · 2019Article
- Aptamers in Education: Undergraduates Make Aptamers and Acquire 21st Century Skills Along the Way.Sensors (Basel, Switzerland) · 2019Article
- Direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of Wolbachia using a cellphone.PLoS neglected tropical diseases · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have found that the overproduction of enzymes in bacteria followed by their lyophilization leads to 'cellular reagents' that can be directly used to carry out numerous molecular biology reactions. We demonstrate the use of cellular reagents in a variety of molecular diagnostics, such as TaqMan qPCR with no diminution in sensitivity, and in synthetic biology cornerstones such as the Gibson assembly of DNA fragments, where new plasmids can be constructed solely based on adding cellular reagents. Cellular reagents have significantly reduced complexity and cost of production, storage and implementation, features that should facilitate accessibility and use in resource-poor conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.