ArticlePloS one2018
Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
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- Fluorescent labeling of proteins in vitro and in vivo using encoded peptide tags.The Journal of biological chemistry · 2025Review
- Rewarding Capacity of Optogenetically Activating a Giant GABAergic Central-Brain Interneuron in LarvalThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Optimized Red-Absorbing Dyes for Imaging and Sensing.Journal of the American Chemical Society · 2023Article
- Cell-Surface Targeting of Fluorophores inACS chemical neuroscience · 2023Article
- A searchable image resource ofeLife · 2023Article
- Sulfonated red and far-red rhodamines to visualize SNAP- and Halo-tagged cell surface proteins.Organic & biomolecular chemistry · 2022Article
- Rest Is Required to Learn an Appetitively-Reinforced Operant Task inFrontiers in behavioral neuroscience · 2021Article
- Voltage Imaging inFrontiers in neuroscience · 2021Article
- Distributed control of motor circuits for backward walking in Drosophila.Nature communications · 2020Article
- A novel temporal identity window generates alternating EveNeural development · 2020Article
- Synthesis of Carboxy ATTO 647N Using Redox Cycling for Xanthone Access.Organic letters · 2020Article
- Mapping Neurotransmitter Identity in the Whole-MountGenetics · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
The use of genetically encoded 'self-labeling tags' with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, Janelia Fluor, Alexa Fluor, and ATTO dyes and tested these with recently improved Drosophila melanogaster transgenes. We found that tissue clearing and mounting in DPX substantially improves signal quality when combined with specific non-cyanine fluorophores. We compared and combined this labeling technique with standard immunohistochemistry in the Drosophila brain.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.