Evidence map›Paper›PMID 30110327›Full record

ArticlePLoS genetics2018

The roles of SMYD4 in epigenetic regulation of cardiac development in zebrafish.

Deyong Xiao, Huijun Wang, Lili Hao, Xiao Guo, Xiaojing Ma, Yanyan Qian, Hongbo Chen, Jing Ma, Jin Zhang, Wei Sheng and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 26 citations in OpenAlex.

  1. Subtype-Specific mInternational journal of molecular sciences · 2026
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  6. Genetic Alterations ofInternational journal of molecular sciences · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 2 countries.

Deyong XiaoKey Laboratory of Metabolism and Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Collaborative Innovation Center of Genetics and Development, Institutes of Biomedical Sciences, School of Basic Medical Sciences, Fudan University, Shanghai, China.ORCID 0000-0002-8353-5582
Huijun WangShanghai Key Lab of Birth Defect, Children's Hospital of Fudan University, Shanghai, China.
Lili HaoKey Laboratory of Metabolism and Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Collaborative Innovation Center of Genetics and Development, Institutes of Biomedical Sciences, School of Basic Medical Sciences, Fudan University, Shanghai, China.
Xiao GuoShanghai Key Lab of Birth Defect, Children's Hospital of Fudan University, Shanghai, China.
Xiaojing MaShanghai Key Lab of Birth Defect, Children's Hospital of Fudan University, Shanghai, China.
Yanyan QianShanghai Key Lab of Birth Defect, Children's Hospital of Fudan University, Shanghai, China.
Hongbo ChenShanghai Key Lab of Birth Defect, Children's Hospital of Fudan University, Shanghai, China.
Jing MaShanghai Key Lab of Birth Defect, Children's Hospital of Fudan University, Shanghai, China.
Jin ZhangKey Laboratory of Metabolism and Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Collaborative Innovation Center of Genetics and Development, Institutes of Biomedical Sciences, School of Basic Medical Sciences, Fudan University, Shanghai, China.
Wei ShengShanghai Key Lab of Birth Defect, Children's Hospital of Fudan University, Shanghai, China.
Weinian ShouCardiovascular Developmental Biology Group, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, United States of America.
Guoying HuangShanghai Key Lab of Birth Defect, Children's Hospital of Fudan University, Shanghai, China.
Duan MaKey Laboratory of Metabolism and Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Collaborative Innovation Center of Genetics and Development, Institutes of Biomedical Sciences, School of Basic Medical Sciences, Fudan University, Shanghai, China.
Children's Hospital of Fudan University · CNFudan University · CNIndiana University School of Medicine

Funding

Transcriptional regulation of cardiac morphogenesisP01HL134599 · NHLBI · INDIANA UNIVERSITY INDIANAPOLIS · PI Anthony B. Firulli · 2017 to 2026
$25.7M
Transcriptional regulation of cardiac conduction system morphogenesisR01HL145060 · NHLBI · INDIANA UNIVERSITY INDIANAPOLIS · PI FIRULLI, ANTHONY B., SHOU, WEINIAN · 2019 to 2022
$2.8M
NHLBI NIH HHS P01 HL134599NHLBI NIH HHS R01 HL145060
6 · The paper itself

Abstract

SMYD4 belongs to a family of lysine methyltransferases. We analyzed the role of smyd4 in zebrafish development by generating a smyd4 mutant zebrafish line (smyd4L544Efs*1) using the CRISPR/Cas9 technology. The maternal and zygotic smyd4L544Efs*1 mutants demonstrated severe cardiac malformations, including defects in left-right patterning and looping and hypoplastic ventricles, suggesting that smyd4 was critical for heart development. Importantly, we identified two rare SMYD4 genetic variants in a 208-patient cohort with congenital heart defects. Both biochemical and functional analyses indicated that SMYD4(G345D) was pathogenic. Our data suggested that smyd4 functions as a histone methyltransferase and, by interacting with HDAC1, also serves as a potential modulator for histone acetylation. Transcriptome and bioinformatics analyses of smyd4L544Efs*1 and wild-type developing hearts suggested that smyd4 is a key epigenetic regulator involved in regulating endoplasmic reticulum-mediated protein processing and several important metabolic pathways in developing zebrafish hearts.

Indexed as

Epigenesis, GeneticAdolescentAnimalsChildChild, PreschoolCohort StudiesCRISPR-Cas SystemsDisease Models, AnimalEmbryonic DevelopmentExome SequencingFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalHeartHeart Defects, CongenitalHistone Deacetylase 1HDAC1 protein, zebrafishHistone Deacetylase 1Histone-Lysine N-MethyltransferaseHistone MethyltransferasesSmyd4 protein, zebrafishZebrafish Proteins

Identifiers

PMID30110327
PMCPMC6110521
OpenAlexW2887008599

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.