ArticleJournal of natural products2018
Complete Genome of Micromonospora sp. Strain B006 Reveals Biosynthetic Potential of a Lake Michigan Actinomycete.
Article in Journal of natural products, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
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- Diversity and taxonomic distribution of bacterial biosynthetic gene clusters predicted to produce compounds with therapeutically relevant bioactivities.Journal of industrial microbiology & biotechnology · 2023Article
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- In Silico Analysis of PKS and NRPS Gene Clusters in Arisostatin- and Kosinostatin-Producers and Description ofAntibiotics (Basel, Switzerland) · 2021Article
- Heterologous expression of the diazaquinomycin biosynthetic gene cluster.Journal of industrial microbiology & biotechnology · 2019Article
- Draft Genome Sequences of Three Sponge-Associated Actinomycetes Exhibiting Antimycobacterial Activity.Microbiology resource announcements · 2019Article
- Omics Technologies to Understand Activation of a Biosynthetic Gene Cluster in Micromonospora sp. WMMB235: Deciphering Keyicin Biosynthesis.ACS chemical biology · 2019Article
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- Diazaquinomycin Biosynthetic Gene Clusters from Marine and Freshwater Actinomycetes.Journal of natural products · 2019Article
- Natural product drug discovery in the genomic era: realities, conjectures, misconceptions, and opportunities.Journal of industrial microbiology & biotechnology · 2019Article
- Heterologous expression of a putative ClpC chaperone gene leads to induction of a host metabolite.Journal of the Brazilian Chemical Society · 2019Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Actinomycete bacteria isolated from freshwater environments are an unexplored source of natural products. Here we report the complete genome of the Great Lakes-derived Micromonospora sp. strain B006, revealing its potential for natural product biosynthesis. The 7-megabase pair chromosome of strain B006 was sequenced using Illumina and Oxford Nanopore technologies followed by Sanger sequencing to close remaining gaps. All identified biosynthetic gene clusters (BGCs) were manually curated. Five known BGCs were identified encoding desferrioxamine, alkyl- O-dihydrogeranylmethoxyhydroquinone, a spore pigment, sioxanthin, and diazepinomicin, which is currently in phase II clinical trials to treat Phelan-McDermid syndrome and co-morbid epilepsy. We report here that strain B006 is indeed a producer of diazepinomicin and at yields higher than previously reported. Moreover, 11 of the 16 identified BGCs are orphan, eight of which were transcriptionally active under the culture condition tested. Orphan BGCs include an enediyne polyketide synthase and an uncharacteristically large, 36-module polyketide synthase-nonribosomal peptide synthetase BGC. We developed a genetics system for Micromonospora sp. B006 that will contribute to deorphaning BGCs in the future. This study is one of the few attempts to report the biosynthetic capacity of a freshwater-derived actinomycete and highlights this resource as a potential reservoir for new natural products.
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