Evidence map›Paper›PMID 30109416›Full record

ArticleParasitology research2018

A Leishmania-specific gene upregulated at the amastigote stage is crucial for parasite survival.

Kumar Avishek, Kavita Ahuja, Dibyabhaba Pradhan, Sreenivas Gannavaram, Angamuthu Selvapandiyan, Hira L Nakhasi, Poonam Salotra

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Article in Parasitology research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Kumar AvishekMolecular Parasitology, National Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, India.
Kavita AhujaJamia Hamdard-Institute of Molecular Medicine, Jamia Hamdard, New Delhi, India.
Dibyabhaba PradhanMolecular Parasitology, National Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, India.
Sreenivas GannavaramDivision of Emerging and Transfusion Transmitted Diseases, CBER, FDA, Bethesda, MD, USA.
Angamuthu SelvapandiyanJamia Hamdard-Institute of Molecular Medicine, Jamia Hamdard, New Delhi, India.
Hira L NakhasiDivision of Emerging and Transfusion Transmitted Diseases, CBER, FDA, Bethesda, MD, USA.
Poonam SalotraMolecular Parasitology, National Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, India. salotra@vsnl.com.
National Institute of Pathology · INInstitute of Molecular Medicine · IN

Funding

Department fo Biotechnology(DBT), Government of India BT/PR441/MED/15/7/2011
6 · The paper itself

Abstract

The morphological and biochemical alterations between the two life stages of Leishmania are governed by stage-regulated expression of several genes. Amastigote-specific genes are believed to have a role in the survival and replication of the parasite in the hostile environment of the mammalian host. Previously, we have reported the upregulated expression of A1 gene (LdA1) at the amastigote stage at RNA level. In the present study, we have further characterized LdA1, in order to understand its role in Leishmania. Sequence homology search revealed that LdA1 is unique to the Leishmania genus. Sequence- and structure-level functional annotations predicted the involvement of LdA1 in a range of biological processes critical for survival of the parasites. Western blot confirmed the upregulated expression of LdA1 at the protein level at the amastigote stage. Overexpression of LdA1 in Leishmania did not affect its growth, phenotype, or infectivity. Attempts to generate null mutants of LdA1 by homologous recombination were not successful. Repeated inability to create null mutants of LdA1 was suggestive of gene essentiality. Mutant parasites with a single allele deletion of LdA1 (LdA1

Indexed as

AnimalsBlotting, WesternHumansLeishmaniaLeishmaniasisLife Cycle StagesMacrophagesProtozoan ProteinsTranscriptional ActivationUp-RegulationProtozoan ProteinsAmastigote-specific geneGene-deleted mutantsLeishmaniaLeishmania-specific geneLive-attenuated parasitesMacrophage infectivity

Identifiers

PMID30109416
OpenAlexW2886219973

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.