ArticleLab on a chip2018
Microfluidic-enabled quantitative measurements of insulin release dynamics from single islets of Langerhans in response to 5-palmitic acid hydroxy stearic acid.
Article in Lab on a chip, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 62 citations in OpenAlex.
- Label-Free Optical Sensor for Real-Time Monitoring of Insulin Secretion from Single Human Pancreatic Islets.Sensors (Basel, Switzerland) · 2026Article
- "Islet-on-a-Chip": A Microfluidic System Design for Preservation of Viability and Function of Human Islets of Langerhans - A Systematic Review.Visceral medicine · 2026Article
- Bubble Perfusion Brain Slice Culture with Single-Droplet Stimulus Delivery in a 3D Printed Microfluidic Device.ACS measurement science au · 2025Article
- Evaluation of fused deposition modeling (FDM)-printed devices for microfluidic-based cell culture studies.Analytical and bioanalytical chemistry · 2025Article
- Investigating the Pathogenesis and Treatment of Type 2 Diabetes from the Perspective of Adipose Tissue.Diabetes, metabolic syndrome and obesity : targets and therapy · 2025Review
- Unveiling islet heterogeneity using an automated microfluidic imaging system.Scientific reports · 2024Article
- Comparison between capillary electrophoresis and fluorescence anisotropy competitive immunoassay for glucagon.Electrophoresis · 2024Article
- Insulin C-peptide secretion on-a-chip to measure the dynamics of secretion and metabolism from individual islets.Cell reports methods · 2023Article
- Adipose tissue-derived lipokines in metabolism.Current opinion in genetics & development · 2023Review
- Automated cellular stimulation with integrated pneumatic valves and fluidic capacitors.The Analyst · 2023Article
- Monitoring hormone and small molecule secretion dynamics from islets-on-chip.Analytical and bioanalytical chemistry · 2023Article
- PAHSAs reduce cellular senescence and protect pancreatic beta cells from metabolic stress through regulation of Mdm2/p53.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Increasing insulin measurement throughput by fluorescence anisotropy imaging immunoassays.Analytica chimica acta · 2022Article
- Investigation of the Therapeutic Potential of New Antidiabetic Compounds Using Islet-on-a-Chip Microfluidic Model.Biosensors · 2022Article
- Islet-on-a-chip for the study of pancreatic β-cell function.In vitro models · 2022Review
- Smartphone-microfluidic fluorescence imaging system for studying islet physiology.Frontiers in endocrinology · 2022Article
- The Foundation for Engineering a Pancreatic Islet Niche.Frontiers in endocrinology · 2022Review
- A microfluidic system for monitoring glucagon secretion from human pancreatic islets of Langerhans.Analytical methods : advancing methods and applications · 2021Article
- Long-term intake of 9-PAHPA or 9-OAHPA modulates favorably the basal metabolism and exerts an insulin sensitizing effect in obesogenic diet-fed mice.European journal of nutrition · 2021Article
- Insulin action in adipocytes, adipose remodeling, and systemic effects.Cell metabolism · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Proper release of insulin from pancreatic islets of Langerhans is essential for maintaining glucose homeostasis. For full efficacy, both the pattern and the amount of hormone release are critical. It is therefore important to understand how insulin levels are secreted from single islets in both a quantitative fashion and in a manner that resolves temporal dynamics. In this study, we describe a microfluidic analytical system that can both quantitatively monitor insulin secretion from single islets while simultaneously maintaining high temporal sampling to resolve dynamics of release. We have applied this system to determine the acute and chronic effects of a recently-identified lipid, 5-palmitic acid hydroxy stearic acid (5-PAHSA), which is a member of the fatty acid hydroxy fatty acid class of lipids that are upregulated in healthy individuals. Chronic incubation (48 h) with 5-PAHSA significantly increased glucose-stimulated insulin secretion (GSIS) in murine islets compared to chronic incubation without the lipid or in the presence of palmitic acid (PA). The studies were continued in human islets from both healthy donors and donors diagnosed with type 2 diabetes mellitus (T2DM). Total amounts of GSIS were not only augmented in islets that were chronically incubated with 5-PAHSA, but the dynamic insulin release profiles also improved as noted by more pronounced insulin oscillations. With this quantitative microfluidic system, we have corroborated the anti-diabetic effects of 5-PAHSA by demonstrating improved islet function after chronic incubation with this lipid via improved oscillatory dynamics along with higher basal and peak release rates.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.