ArticleNature communications2018
Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Circadian temperature compensation is intrinsically linked with metabolism and redox signaling.Nature communications · 2026Article
- Cyanobacterial Circadian Clock: Molecular Mechanisms and Physiological Outputs.Plants (Basel, Switzerland) · 2026Review
- Physical Frameworks and Theories on How Autonomous Biological Oscillators Synchronize.Advances in experimental medicine and biology · 2026Review
- The priming phosphorylation of KaiC is activated by the release of its autokinase autoinhibition.PNAS nexus · 2025Article
- Programming scheduled self-assembly of circadian materials.Nature communications · 2025Article
- A topological mechanism for robust and efficient global oscillations in biological networks.Nature communications · 2024Article
- Article
- The inner workings of an ancient biological clock.Trends in biochemical sciences · 2024Review
- Article
- The Case for the Target of Rapamycin Pathway as a Candidate Circadian Oscillator.International journal of molecular sciences · 2023Review
- Role of the reaction-structure coupling in temperature compensation of the KaiABC circadian rhythm.PLoS computational biology · 2022Article
- Article
- Regulation mechanisms of the dual ATPase in KaiC.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Overall structure of fully assembled cyanobacterial KaiABC circadian clock complex by an integrated experimental-computational approach.Communications biology · 2022Article
- Evolution of the repression mechanisms in circadian clocks.Genome biology · 2022Article
- Shift in Conformational Equilibrium Underlies the Oscillatory Phosphoryl Transfer Reaction in the Circadian Clock.Life (Basel, Switzerland) · 2021Article
- The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization.BMC biology · 2021Article
- Mechanism of autonomous synchronization of the circadian KaiABC rhythm.Scientific reports · 2021Article
- Spectres of Clock Evolution: Past, Present, and Yet to Come.Frontiers in physiology · 2021Review
- Bayesian modeling reveals metabolite-dependent ultrasensitivity in the cyanobacterial circadian clock.Molecular systems biology · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The circadian clock proteins KaiA, KaiB, and KaiC reconstitute a remarkable circa-24 h oscillation of KaiC phosphorylation that persists for many days in vitro. Here we use high-speed atomic force microscopy (HS-AFM) to visualize in real time and quantify the dynamic interactions of KaiA with KaiC on sub-second timescales. KaiA transiently interacts with KaiC, thereby stimulating KaiC autokinase activity. As KaiC becomes progressively more phosphorylated, KaiA's affinity for KaiC weakens, revealing a feedback of KaiC phosphostatus back onto the KaiA-binding events. These non-equilibrium interactions integrate high-frequency binding and unbinding events, thereby refining the period of the longer term oscillations. Moreover, this differential affinity phenomenon broadens the range of Kai protein stoichiometries that allow rhythmicity, explaining how the oscillation is resilient in an in vivo milieu that includes noise. Therefore, robustness of rhythmicity on a 24-h scale is explainable by molecular events occurring on a scale of sub-seconds.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.