ArticleVirology journal2018
Localization of cassava brown streak virus in Nicotiana rustica and cassava Manihot esculenta (Crantz) using RNAscope® in situ hybridization.
Article in Virology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 16 citations in OpenAlex.
- Mixed-Pathogen Infections in Vegetatively Propagated Crops: From Biological Synergism to Integrated Management.Plants (Basel, Switzerland) · 2026Review
- Automated In situ Hybridization for Detection of Viral Pathogens Using RNAscope™.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Isolation, genome analysis and tissue localization of Ceratobasidium theobromae, a new encounter pathogen of cassava in Southeast Asia.Scientific reports · 2024Article
- OMICs, Epigenetics, and Genome Editing Techniques for Food and Nutritional Security.Plants (Basel, Switzerland) · 2021Review
- Article
- Visualization of spatial gene expression in plants by modified RNAscope fluorescent in situ hybridization.Plant methods · 2020Article
- Expanded application to plant reproductive tissues of a branched DNA probe-based in situ hybridization method.Applications in plant sciencesArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 4 countries.
Funding
Abstract
backgroundCassava brown streak disease (CBSD) has a viral aetiology and is caused by viruses belonging to the genus Ipomovirus (family Potyviridae), Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Molecular and serological methods are available for detection, discrimination and quantification of cassava brown streak viruses (CBSVs) in infected plants. However, precise determination of the viral RNA localization in infected host tissues is still not possible pending appropriate methods.
resultsWe have developed an in situ hybridization (ISH) assay based on RNAscope® technology that allows the sensitive detection and localization of CBSV RNA in plant tissues. The method was initially developed in the experimental host Nicotiana rustica and was then further adapted to cassava. Highly sensitive and specific detection of CBSV RNA was achieved without background and hybridization signals in sections prepared from non-infected tissues. The tissue tropism of CBSV RNAs appeared different between N. rustica and cassava.
conclusionsThis study provides a robust method for CBSV detection in the experimental host and in cassava. The protocol will be used to study CBSV tropism in various cassava genotypes, as well as CBSVs/cassava interactions in single and mixed infections.
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Registered trials
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