ArticleGenome biology2018
QsRNA-seq: a method for high-throughput profiling and quantifying small RNAs.
Article in Genome biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Cargo molecules in plasma neural-derived extracellular vesicles reveal pathological signatures of primary open-angle glaucoma.Journal of translational medicine · 2026Article
- Co-transcriptional folding orchestrates sequential multi-effector sensing by a glycine tandem riboswitch.Nature communications · 2026Article
- Identification of Salivary Exosome-Derived miRNAs as Potential Biomarkers for Non-Invasive Diagnosis and Proactive Monitoring of Inflammatory Bowel Disease.International journal of molecular sciences · 2025Article
- Sequential structure probing of cotranscriptional RNA folding intermediates.Nature communications · 2025Article
- ChEC-Seq: A Comprehensive Guide for Scalable and Cost-Efficient Genome-Wide Profiling in Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Observation of coordinated RNA folding events by systematic cotranscriptional RNA structure probing.Nature communications · 2023Article
- Unlocking the potential of non-coding RNAs in cancer research and therapy.Translational oncology · 2023Article
- Circulating microRNAs in seminal plasma as predictors of sperm retrieval in microdissection testicular sperm extraction.Annals of translational medicine · 2022Article
- Accumulation of cis- and trans-regulatory variations is associated with phenotypic divergence of a complex trait between yeast species.G3 (Bethesda, Md.) · 2021Article
- Mechanical loading stimulates bone angiogenesis through enhancing type H vessel formation and downregulating exosomal miR-214-3p from bone marrow-derived mesenchymal stem cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Circulating exosomal small RNAs are promising non-invasive diagnostic biomarkers for gastric cancer.Journal of cellular and molecular medicine · 2020Article
- Article
- Simultaneous RNA purification and size selection using on-chip isotachophoresis with an ionic spacer.Lab on a chip · 2019Article
- QsRNA-seq: A protocol for generating libraries for high-throughput sequencing of small RNAs.Bio-protocol · 2019Article
- QsRNA-seq: a method for high-throughput profiling and quantifying small RNAs.Genome biology · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The ability to profile and quantify small non-coding RNAs (sRNAs), specifically microRNAs (miRNAs), using high-throughput sequencing is challenging because of their small size. We developed QsRNA-seq, a method for preparation of sRNA libraries for high-throughput sequencing that overcomes this difficulty by enabling a gel-free separation of fragments shorter than 100 nt that differ only by 20 nt in length. The method allows the use of unique molecular identifiers for quantification and is more amenable to automation than gel-based methods. We show that QsRNA-seq gives very accurate, comprehensive, and reproducible results by looking at miRNAs in Caenorhabditis elegans embryos and larvae.
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Registered trials
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