Evidence map›Paper›PMID 30106247›Full record

ArticleBrain and behavior2018

miRNA-regulated transcription associated with mouse strains predisposed to hypnotic effects of ethanol.

B Vestal, P Russell, R A Radcliffe, L Bemis, L M Saba, K Kechris

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brain and behavior, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

B VestalCenter for Genes, Environment and Health, National Jewish Health, Denver, Colorado.ORCID 0000-0002-3772-1691
P RussellDepartment of Biostatistics and Informatics, University of Colorado Denver Anschutz Medical Campus, Aurora, Colorado.
R A RadcliffeDepartment of Pharmaceutical Sciences, University of Colorado Denver Anschutz Medical Campus, Aurora, Colorado.
L BemisDepartment of Biomedical Sciences, University of Minnesota Medical School Duluth Campus, Duluth, Minnesota.
L M SabaDepartment of Pharmaceutical Sciences, University of Colorado Denver Anschutz Medical Campus, Aurora, Colorado.
K KechrisDepartment of Biostatistics and Informatics, University of Colorado Denver Anschutz Medical Campus, Aurora, Colorado.
Colorado School of Public Health · USUniversity of Colorado Anschutz Medical Campus · USUniversity of Minnesota, Duluth · US

Funding

The Heritable Transcriptome and AlcoholismR24AA013162 · NIAAA · UNIVERSITY OF COLORADO DENVER · PI HOFFMAN, PAULA, SABA, LAURA MAREN · 2001 to 2021
$14.2M
TIM Project NIDA P30 CenterP30DA044223 · NIDA · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI SABA, LAURA MAREN, WILLIAMS, ROBERT W. · 2017 to 2021
$3.9M
Genetic Studies of Alcohol ToleranceR01AA016957 · NIAAA · UNIVERSITY OF COLORADO DENVER · PI RADCLIFFE, RICHARD A · 2009 to 2013
$2.5M
Genome-wide identification of miRNAs associated with alcoholism endophenotypesR01AA021131 · NIAAA · UNIVERSITY OF COLORADO DENVER · PI KECHRIS-MAYS, KATHERINA · 2013 to 2017
$1.6M
NIAAA NIH HHS R01 AA016957NIAAA NIH HHS R01 AA021131NIAAA NIH HHS R24 AA013162NIDA NIH HHS P30 DA044223
6 · The paper itself

Abstract

introductionStudying innate sensitivity to ethanol can be an important first step toward understanding alcohol use disorders. In brain, we investigated transcripts, with evidence of miRNA modulation related to a predisposition to the hypnotic effect of ethanol, as measured by loss of righting reflex (LORR).

methodsExpression of miRNAs (12 samples) and expression of mRNAs (353 samples) in brain were independently analyzed for an association with LORR in mice from the LXS recombinant inbred panel gathered across several small studies. These results were then integrated via a meta-analysis of miRNA-mRNA target pairs identified in miRNA-target interaction databases.

resultsWe found 112 significant miRNA-mRNA pairs where a large majority of miRNAs and mRNAs were highly interconnected. Most pairs indicated a pattern of increased levels of miRNAs and reduced levels of mRNAs being associated with more alcohol-sensitive strains. For example, CaMKIIn1 was targeted by multiple miRNAs associated with LORR. CAMK2N1 is an inhibitor of CAMK2, which among other functions, phosphorylates, or binds to GABA

conclusionsOur results suggest a novel role of miRNA-mediated regulation of an inhibitor of CAMK2 and its downstream targets including the GABA

Indexed as

AlcoholismAnimalsCalcium-Calmodulin-Dependent Protein Kinase Type 2EthanolFemaleGABA-A Receptor AntagonistsGene Expression RegulationGenetic Predisposition to DiseaseHypnotics and SedativesMiceMice, Inbred StrainsMicroRNAsReceptors, GABA-AReceptors, N-Methyl-D-AspartateReflex, RightingRNA, MessengerCalcium-Calmodulin-Dependent Protein Kinase Type 2Camk2a protein, mouseEthanolGABA-A Receptor AntagonistsHypnotics and SedativesMicroRNAsReceptors, GABA-AReceptors, N-Methyl-D-AspartateRNA, Messengercalcium/calmodulin‐dependent protein kinase II inhibitor 1ethanol sensitivityloss of righting reflexmicroRNA expressionmicroRNA target interactionsmmu‐miR‐106bmouse brainmRNA expressionrecombinant Inbred mouse strainsRNA sequencing

Identifiers

PMID30106247
PMCPMC5991579
OpenAlexW2800974848

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.