ArticleMicrobial biotechnology2018
Insight into metabolic diversity of the brown-rot basidiomycete Postia placenta responsible for sesquiterpene biosynthesis: semi-comprehensive screening of cytochrome P450 monooxygenase involved in protoilludene metabolism.
Article in Microbial biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Integration of pathway balance and protein fusion enables de novo biosynthesis of (+)-bicyclogermacrene in Escherichia coli.Bioresources and bioprocessing · 2026Article
- Characterization of the Cubamyces Menziesii Terpenome.Chembiochem : a European journal of chemical biology · 2025Article
- Cloning and functional characterization of sesquiterpene synthase genes from Inonotus obliquus using a Saccharomyces cerevisiae expression system.World journal of microbiology & biotechnology · 2025Article
- The Recent Progress of Tricyclic Aromadendrene-Type Sesquiterpenoids: Biological Activities and Biosynthesis.Biomolecules · 2024Review
- Genome Mining of Fungal Unique Trichodiene Synthase-like Sesquiterpene Synthases.Journal of fungi (Basel, Switzerland) · 2024Article
- Molecular and Functional Analyses of Characterized Sesquiterpene Synthases in Mushroom-Forming Fungi.Journal of fungi (Basel, Switzerland) · 2023Article
- Biodegradation of Benzo[a]pyrene by a White-Rot FungusJournal of fungi (Basel, Switzerland) · 2023Article
- An HMM approach expands the landscape of sesquiterpene cyclases across the kingdom Fungi.Microbial genomics · 2023Article
- Biocatalyst collection and heterologous expression of sesquiterpene synthases from basidiomycetous fungi: Discovery of a novel sesquiterpene hydrocarbon.Microbial biotechnology · 2023Article
- Chromosome-Level Genome Sequences, Comparative Genomic Analyses, and Secondary-Metabolite Biosynthesis Evaluation of the Medicinal Edible Mushroom Laetiporus sulphureus.Microbiology spectrum · 2022Article
- Biosynthesis of Sesquiterpenes in Basidiomycetes: A Review.Journal of fungi (Basel, Switzerland) · 2022Review
- Ectomycorrhizal Influence on the Dynamics of Sesquiterpene Release byJournal of fungi (Basel, Switzerland) · 2022Article
- Article
- Characterization of γ-Cadinene Enzymes inACS omega · 2022Article
- Effects of Culture Mechanism ofMycobiology · 2022Article
- Sesquiterpenoids Specially Produced by Fungi: Structures, Biological Activities, Chemical and Biosynthesis (2015-2020).Journal of fungi (Basel, Switzerland) · 2021Review
- Biosynthesis and regulation of terpenoids from basidiomycetes: exploration of new research.AMB Express · 2021Review
- Identification of a self-sufficient cytochrome P450 monooxygenase from Cupriavidus pinatubonensis JMP134 involved in 2-hydroxyphenylacetic acid catabolism, via homogentisate pathway.Microbial biotechnology · 2021Article
- Bioinformatics-aided identification, characterization and applications of mushroom linalool synthases.Communications biology · 2021Article
- RNA-Seq de Novo Assembly and Differential Transcriptome Analysis of Chaga (International journal of molecular sciences · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A wide variety of sesquiterpenoids have been isolated from basidiomycetes, and their bioactive properties have attracted significant attention in an effort to understand biosynthetic machineries. As both sesquiterpene synthases and cytochrome P450 monooxygenases play key roles in the diversification of sesquiterpenoids, it is important to widely and mutually understand their biochemical properties. In this study, we performed genome-wide annotation and functional characterization of sesquiterpene synthases from the brown-rot basidiomycete Postia placenta. Using RT-PCR, we isolated 16 sesquiterpene synthases genes as full-length cDNAs. Heterologous expression revealed that the sesquiterpene synthases could produce a series of sesquiterpene scaffolds with distinct metabolic profiles. Based on metabolic studies, we identified 25 sesquiterpene scaffolds including Δ6-protoilludene produced by the sesquiterpene synthases. In particular, a protoilludene synthase from the brown-rot basidiomycete was characterized for the first time. Furthermore, we conducted a semi-comprehensive functional screening of cytochrome P450 monooxygenases from P. placenta to elucidate biosynthetic machineries involved in metabolisms of Δ6-protoilludene. Coexpression of protoilludene synthase and 184 isoforms of cytochrome P450 monooxygenases enabled the identification of CYP5344B1, CYP5348E1 and CYP5348J3, which catalysed the hydroxylation reaction of Δ6-protoilludene to produce Δ6-protoilludene-8-ol and Δ6-protoilludene-5-ol. Furthermore, structural isomers of Δ7-protoilludene-6-ol were obtained from incubation of Δ6-protoilludene-8-ol in acidic culture medium.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.