Evidence map›Paper›PMID 30105406›Full record

ArticleParasitology research2018

In vitro expression and functional characterization of NPA motifs in aquaporins of Nosema bombycis.

Gong Chen, Zhilin Zhang, Ruisha Shang, Jingru Qi, Yiling Zhang, Shunming Tang, Zhongyuan Shen

Abstract read
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In one paragraph

Article in Parasitology research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Germination of Microsporidian Spores: The Known and Unknown.Journal of fungi (Basel, Switzerland) · 2023
    Review
  2. dbAQP-SNP: a database of missense single-nucleotide polymorphisms in human aquaporins.Database : the journal of biological databases and curation · 2023
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Gong ChenJiangsu University of Science and Technology, Zhenjiang, 212018, Jiangsu Province, China.
Zhilin ZhangJiangsu University of Science and Technology, Zhenjiang, 212018, Jiangsu Province, China.
Ruisha ShangJiangsu University of Science and Technology, Zhenjiang, 212018, Jiangsu Province, China.
Jingru QiJiangsu University of Science and Technology, Zhenjiang, 212018, Jiangsu Province, China.
Yiling ZhangJiangsu University of Science and Technology, Zhenjiang, 212018, Jiangsu Province, China.
Shunming TangJiangsu University of Science and Technology, Zhenjiang, 212018, Jiangsu Province, China.
Zhongyuan ShenJiangsu University of Science and Technology, Zhenjiang, 212018, Jiangsu Province, China. szysri@163.com.ORCID http://orcid.org/0000-0003-1904-1796
Jiangsu University of Science and Technology · CNJiangsu University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nosema bombycis contains functional aquaporins (NbAQPs), which are key targets for exploring the mechanism of N. bombycis infection; however, the regulation of these NbAQPs remains unknown. The two highly conserved asparagine-proline-alanine sequences (NPA motifs) play important roles in AQP biogenesis. As part of this study, we constructed a series of NbAQP mutants (NbAQP_NPA1, NbAQP_NPA2, and NbAQP_NPA1,2) and expressed them in BmN cells. The results showed that mutations in either NPA motif or in both NPA motifs did not affect NbAQP expression in vitro. After expression in Xenopus laevis oocytes, those injected with wild-type NbAQP rapidly expanded, whereas oocytes injected with NbAQP_NPAs did not significantly change in size. The associated water permeability (pf) of NbAQP_NPAs was significantly reduced five-six times compared to that of wild-type NbAQP. These results indicated that NPA motifs are necessary for the water channel function of AQPs in N. bombycis. The present study shows for the first time that the NbAQP NPA motif has an impact on the water permeability of aquaporin in N. bombycis, thereby providing a platform for further research into the mechanisms underlying the regulation of NbAQP expression.

Indexed as

Amino Acid SequenceAnimalsAquaporinsConserved SequenceFemaleNosemaOligopeptidesOocytesWaterXenopus laevisAquaporinsasparagine-proline-alanineOligopeptidesWaterAquaporinImmunofluorescenceNosema bombycisNPA motifsWater permeability

Identifiers

PMID30105406
OpenAlexW2885747783

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.