Evidence map›Paper›PMID 30105179›Full record

Article3 Biotech2018

A simple and inexpensive physical lysis method for DNA and RNA extraction from freshwater microalgae.

Willy Yee, Ruzanna Abdul-Kadir, Leng May Lee, Benson Koh, Ying Shi Lee, Heng Yee Chan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in 3 Biotech, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Identification and inoculation of fungal strains fromSaudi journal of biological sciences · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Willy YeeDepartment of Biosciences, Faculty of Applied Sciences, Nilai University, No. 1, Persiaran Universiti, 71800 Nilai, Negeri Sembilan Malaysia.ORCID 0000-0001-6739-2000
Ruzanna Abdul-KadirDepartment of Biosciences, Faculty of Applied Sciences, Nilai University, No. 1, Persiaran Universiti, 71800 Nilai, Negeri Sembilan Malaysia.
Leng May LeeDepartment of Biosciences, Faculty of Applied Sciences, Nilai University, No. 1, Persiaran Universiti, 71800 Nilai, Negeri Sembilan Malaysia.
Benson KohDepartment of Biosciences, Faculty of Applied Sciences, Nilai University, No. 1, Persiaran Universiti, 71800 Nilai, Negeri Sembilan Malaysia.
Ying Shi LeeDepartment of Biosciences, Faculty of Applied Sciences, Nilai University, No. 1, Persiaran Universiti, 71800 Nilai, Negeri Sembilan Malaysia.
Heng Yee ChanDepartment of Biosciences, Faculty of Applied Sciences, Nilai University, No. 1, Persiaran Universiti, 71800 Nilai, Negeri Sembilan Malaysia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this work, a simple and inexpensive physical lysis method using a cordless drill fitted with a plastic pellet pestle and 150 mg of sterile sea sand was established for the extraction of DNA from six strains of freshwater microalgae. This lysis method was also tested for RNA extraction from two microalgal strains. Lysis duration between 15 and 120 s using the cetyltrimethyl ammonium bromide (CTAB) buffer significantly increased the yield of DNA from four microalgalstrains (

Indexed as

DNA extractionMicroalgaeNucleic acidPhysical lysisRNA extractionSea sand

Identifiers

PMID30105179
PMCPMC6070457

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.