ArticleScientific reports2018
Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Cold, Hot, and Lethal-The Tumour Microenvironment and the Immunology of Head and Neck Squamous Cell Carcinoma.International journal of molecular sciences · 2025Review
- Capturing cell morphology dynamics with high temporal resolution using single-shot quantitative phase gradient imaging.Journal of biomedical optics · 2024Article
- Morphological entropy encodes cellular migration strategies on multiple length scales.NPJ systems biology and applications · 2024Article
- Suppression of the growth and metastasis of mouse melanoma byFrontiers in immunology · 2024Article
- Quantitative phase imaging for characterization of single cell growth dynamics.Lasers in medical science · 2023Review
- Changes in cell surface excess are coordinated with protrusion dynamics during 3D motility.Biophysical journal · 2023Article
- Dry mass photometry of single bacteria using quantitative wavefront microscopy.Biophysical journal · 2023Article
- Plasticity of cancer invasion and energy metabolism.Trends in cell biology · 2023Review
- Folylpolyglutamate synthetase mRNA G-quadruplexes regulate its cell protrusion localization and enhance a cancer cell invasive phenotype upon folate repletion.BMC biology · 2023Article
- The emerging role of microtubules in invasion plasticity.Frontiers in oncology · 2023Review
- Biomass measurements of single neuritesBiomedical optics express · 2022Article
- Low-intensity illumination for lensless digital holographic microscopy with minimized sample interaction.Biomedical optics express · 2022Article
- Simple and automatic monitoring of cancer cell invasion into an epithelial monolayer using label-free holographic microscopy.Scientific reports · 2022Article
- Functional imaging with dynamic quantitative oblique back-illumination microscopy.Journal of biomedical optics · 2022Article
- Addressing cancer invasion and cell motility with quantitative light microscopy.Scientific reports · 2022Article
- Cancer cell metabolic plasticity in migration and metastasis.Clinical & experimental metastasis · 2021Review
- Invadopodia Structure in 3D Environment Resolved by Near-Infrared Branding Protocol Combining Correlative Confocal and FIB-SEM Microscopy.International journal of molecular sciences · 2021Article
- Variations of intracellular density during the cell cycle arise from tip-growth regulation in fission yeast.eLife · 2021Article
- Three-dimensional label-free imaging and quantification of migrating cells during wound healing.Biomedical optics express · 2020Article
- Exploiting the potential of commercial digital holographic microscopy by combining it with 3D matrix cell culture assays.Scientific reports · 2020Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Observation and analysis of cancer cell behaviour in 3D environment is essential for full understanding of the mechanisms of cancer cell invasion. However, label-free imaging of live cells in 3D conditions is optically more challenging than in 2D. Quantitative phase imaging provided by coherence controlled holographic microscopy produces images with enhanced information compared to ordinary light microscopy and, due to inherent coherence gate effect, enables observation of live cancer cells' activity even in scattering milieu such as the 3D collagen matrix. Exploiting the dynamic phase differences method, we for the first time describe dynamics of differences in cell mass distribution in 3D migrating mesenchymal and amoeboid cancer cells, and also demonstrate that certain features are shared by both invasion modes. We found that amoeboid fibrosarcoma cells' membrane blebbing is enhanced upon constriction and is also occasionally present in mesenchymally invading cells around constricted nuclei. Further, we demonstrate that both leading protrusions and leading pseudopods of invading fibrosarcoma cells are defined by higher cell mass density. In addition, we directly document bundling of collagen fibres by protrusions of mesenchymal fibrosarcoma cells. Thus, such a non-invasive microscopy offers a novel insight into cellular events during 3D invasion.
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