ArticleScientific reports2018
Effect of storage conditions on salivary polyamines quantified via liquid chromatography-mass spectrometry.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 3 of them syntheses that pooled it.
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Who cites it
23 citing papers in PubMed, 3 syntheses or guidelines pooled it, 42 citations in OpenAlex.
- Salivary metabolites for pancreatic cancer diagnosis: a systematic review and meta-analysis.BMC gastroenterology · 2025Pooled it
- Evaluation of Salivary Biomarkers of Periodontal Disease Based on Smoking Status: A Systematic Review.International journal of environmental research and public health · 2022Pooled it
- Salivary metabolites to detect patients with cancer: a systematic review.International journal of clinical oncology · 2020Pooled it
- Cross-sectional associations between CES-D scores and the salivary metabolome in nonclinical adults.Discover mental health · 2026Article
- Sensitivity Analysis of a New Cancer Risk Assessment Tool That Evaluates Salivary Polyamines.Cureus · 2025Article
- Saliva metabolomics: a non-invasive frontier for diagnosing and managing oral diseases.Journal of translational medicine · 2025Review
- Profiling of metabolic dysregulation in ovarian cancer tissues and biofluids.Scientific reports · 2024Article
- Metabolomic profiling of cancer-related fatigue involved in cachexia and chemotherapy.Scientific reports · 2024Article
- Salivary metabolites as novel independent predictors of radiation pneumonitis.Journal of cancer research and clinical oncology · 2023Article
- Salivary Polyamines Help Detect High-Risk Patients with Pancreatic Cancer: A Prospective Validation Study.International journal of molecular sciences · 2023Article
- Relationship between Urinary Metabolomic Profiles and Depressive Episode in Antarctica.International journal of molecular sciences · 2023Article
- Metabolomics Analysis of Blood, Urine, and Saliva Samples Based on Capillary Electrophoresis-Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2023Article
- One-Carbon and Polyamine Metabolism as Cancer Therapy Targets.Biomolecules · 2022Review
- Salivary metabolomics with machine learning for colorectal cancer detection.Cancer science · 2022Article
- Long-Term Mastication Changed Salivary Metabolomic Profiles.Metabolites · 2022Article
- Salivary Metabolomics for Oral Cancer Detection: A Narrative Review.Metabolites · 2022Review
- Evaluation of Saliva Stability for NMR Metabolomics: Collection and Handling Protocols.Metabolites · 2020Article
- Serum Metabolomic Profiling of Patients with Non-Infectious Uveitis.Journal of clinical medicine · 2020Article
- Quantification of Salivary Charged Metabolites using Capillary Electrophoresis Time-of-flight-mass Spectrometry.Bio-protocol · 2020Article
- Gender-Related Differences on Polyamine Metabolome in Liquid Biopsies by a Simple and Sensitive Two-Step Liquid-Liquid Extraction and LC-MS/MS.Biomolecules · 2019Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salivary polyamines are potential non-invasive tools for screening various types of cancers. For clinical use, the reproducibility of these metabolites should be evaluated under various storage conditions, including duration and temperature, to establish standard operating protocols. Polyamines and amino acids in unstimulated whole saliva were quantified via liquid chromatography-mass spectrometry. Concentrations of time course samples were analysed after short-term storage for up to 240 min and long-term storage for up to 8 days under various storage conditions. As expected, storage at the lowest temperature (-18 °C) exerted the least pronounced effects on the quantified values in both tests. At a higher temperature, polyamines were more stable than amino acids, as evident from polyamine profiling. Addition of ethanol significantly stabilized polyamine profiles even at a higher temperature. Comparative processing of saliva revealed a minor effect of the solvent, whereas drying had a more prominent effect on polyamine profiles. Computational analyses evaluated the ability of polyamines to discriminate pancreatic cancer from controls. Repeated noise added tests were designed on the basis of the results of the storage tests; these analyses confirmed that the discriminative abilities were robust. These data contribute to the standardization of salivary storage conditions, thereby highlighting the clinical utility of saliva.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.