ArticleScientific reports2018
Optimising complementary soft tissue synchrotron X-ray microtomography for reversibly-stained central nervous system samples.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
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- High-resolution imaging and three-dimensional model of the feline spinal cord.Frontiers in neuroanatomy · 2026Article
- Advancements in High-Resolution Computed Tomography: Revolutionising Bone Health Micro-Research.Bioengineering (Basel, Switzerland) · 2025Article
- Air artifact suppression in phase contrast micro-CT using conditional generative adversarial networks.Journal of synchrotron radiation · 2025Article
- Discorpy: algorithms and software for camera calibration and correction.Journal of synchrotron radiation · 2025Article
- Mineral coated microparticles delivering Interleukin-4, Interleukin-10, and Interleukin-13 reduce inflammation and improve function after spinal cord injury in a rat.Experimental neurology · 2025Article
- Advanced soft tissue visualization in conjunction with bone structures using contrast-enhanced micro-CT.Journal of medical imaging (Bellingham, Wash.) · 2024Article
- Multiscale and multimodal imaging for three-dimensional vascular and histomorphological organ structure analysis of the pancreas.Scientific reports · 2024Article
- Preparation of large biological samples for high-resolution, hierarchical, synchrotron phase-contrast tomography with multimodal imaging compatibility.Nature protocols · 2023Review
- Morphological and Chemical Investigation of Ovarian Structures in a Bovine Model by Contrast-Enhanced X-ray Imaging and Microscopy.International journal of molecular sciences · 2023Article
- Article
- Microvascular imaging of the unstained human superior colliculus using synchrotron-radiation phase-contrast microtomography.Scientific reports · 2022Article
- Regional variations in discrete collagen fibre mechanics within intact intervertebral disc resolved using synchrotron computed tomography and digital volume correlation.Acta biomaterialia · 2022Article
- SuRVoS 2: Accelerating Annotation and Segmentation for Large Volumetric Bioimage Workflows Across Modalities and Scales.Frontiers in cell and developmental biology · 2022Article
- Iodine doping of a photocuring, one-component embedding medium for µCT and histology.The British journal of radiology · 2021Article
- A massively multi-scale approach to characterizing tissue architecture by synchrotron micro-CT applied to the human placenta.Journal of the Royal Society, Interface · 2021Article
- A combinatorial method to visualize the neuronal network in the mouse spinal cord: combination of a modified Golgi-Cox method and synchrotron radiation micro-computed tomography.Histochemistry and cell biology · 2021Article
- The spatiotemporal spread of cervical spinal cord contusion injury pathology revealed by 3D in-line phase contrast synchrotron X-ray microtomography.Experimental neurology · 2021Article
- Scalable method for micro-CT analysis enables large scale quantitative characterization of brain lesions and implants.Scientific reports · 2020Article
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8 authors.
Funding
Abstract
Synchrotron radiation microtomography (SRμCT) is a nominally non-destructive 3D imaging technique which can visualise the internal structures of whole soft tissues. As a multi-stage technique, the cumulative benefits of optimising sample preparation, scanning parameters and signal processing can improve SRμCT imaging efficiency, image quality, accuracy and ultimately, data utility. By evaluating different sample preparations (embedding media, tissue stains), imaging (projection number, propagation distance) and reconstruction (artefact correction, phase retrieval) parameters, a novel methodology (combining reversible iodine stain, wax embedding and inline phase contrast) was optimised for fast (~12 minutes), high-resolution (3.2-4.8 μm diameter capillaries resolved) imaging of the full diameter of a 3.5 mm length of rat spinal cord. White-grey matter macro-features and micro-features such as motoneurons and capillary-level vasculature could then be completely segmented from the imaged volume for analysis through the shallow machine learning SuRVoS Workbench. Imaged spinal cord tissue was preserved for subsequent histology, establishing a complementary SRμCT methodology that can be applied to study spinal cord pathologies or other nervous system tissues such as ganglia, nerves and brain. Further, our 'single-scan iterative downsampling' approach and side-by-side comparisons of mounting options, sample stains and phase contrast parameters should inform efficient, effective future soft tissue SRμCT experiment design.
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