ArticleMicrobiome2018
The tick endosymbiont Candidatus Midichloria mitochondrii and selenoproteins are essential for the growth of Rickettsia parkeri in the Gulf Coast tick vector.
Article in Microbiome, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- First molecular detection of Cytauxzoon felis and Babesia sp. in ticks collected from wildlife in Atwemonom bushmeat market of Ghana and their potential veterinary health implications.Veterinary research communications · 2026Article
- Comparative tissue-specific microbiome analyses identify keystone endosymbionts shaping pathogen interactions in dromedary camel ticks.BMC microbiology · 2026Article
- Sexual and regional differences in the microbiome and functional metagenome of the lone star tick, Amblyomma americanum.Animal microbiome · 2025Article
- Vaccination-induced neutralizing antibodies in immunocompetent hosts correlate with protection against rickettsiae.NPJ vaccines · 2025Article
- Tick-Borne Viruses in a Changing Climate: The Expanding Threat in Africa and Beyond.Microorganisms · 2025Review
- Insecticide resistant Anopheles from Ethiopia but not Burkina Faso show a microbiota composition shift upon insecticide exposure.Parasites & vectors · 2025Article
- Differential impact of spotted fever groupFrontiers in microbiology · 2025Article
- Deciphering the microbial communities in ticks of Inner Mongolia: ecological determinants and pathogen profiles.Parasites & vectors · 2024Article
- Ticks and tick-borne pathogens in selected abattoirs and a slaughter slab in Kumasi, Ghana.Veterinary medicine and science · 2024Article
- Infection Rates and Characterisation ofMicroorganisms · 2024Article
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- Ticks without borders: microbiome of immature neotropical tick species parasitizing migratory songbirds along northern Gulf of Mexico.Frontiers in cellular and infection microbiology · 2024Article
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- Key molecules regulating the blood meals ofVeterinary research forum : an international quarterly journal · 2024Article
- Differential associations of horizontally and vertically transmitted symbionts on Ixodes ricinus behaviour and physiology.Parasites & vectors · 2023Article
- Article
- Novel Francisella-like endosymbiont and Anaplasma species from Amblyomma nodosum hosted by the anteater Tamandua Mexicana in Mexico.Experimental & applied acarology · 2023Article
- Comparative Microbiome Analysis of Three Epidemiologically Important Tick Species in Latvia.Microorganisms · 2023Article
- PERK-mediated antioxidant response is key for pathogen persistence in ticks.bioRxiv : the preprint server for biology · 2023Article
- New insights into the impact of microbiome on horizontal and vertical transmission of a tick-borne pathogen.Microbiome · 2023Article
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Authors and funding
6 authors.
Funding
Abstract
backgroundPathogen colonization inside tick tissues is a significant aspect of the overall competence of a vector. Amblyomma maculatum is a competent vector of the spotted fever group rickettsiae, Rickettsia parkeri. When R. parkeri colonizes its tick host, it has the opportunity to dynamically interact with not just its host but with the endosymbionts living within it, and this enables it to modulate the tick's defenses by regulating tick gene expression. The microbiome in A. maculatum is dominated by two endosymbiont microbes: a Francisella-like endosymbiont (FLE) and Candidatus Midichloria mitochondrii (CMM). A range of selenium-containing proteins (selenoproteins) in A. maculatum ticks protects them from oxidative stress during blood feeding and pathogen infections. Here, we investigated rickettsial multiplication in the presence of tick endosymbionts and characterized the functional significance of selenoproteins during R. parkeri replication in the tick.
resultsFLE and CMM were quantified throughout the tick life stages by quantitative PCR in R. parkeri-infected and uninfected ticks. R. parkeri infection was found to decrease the FLE numbers but CMM thrived across the tick life cycle. Our qRT-PCR analysis indicated that the transcripts of genes with functions related to redox (selenogenes) were upregulated in ticks infected with R. parkeri. Three differentially expressed proteins, selenoprotein M, selenoprotein O, and selenoprotein S were silenced to examine their functional significance during rickettsial replication within the tick tissues. Gene silencing of the target genes was found to impair R. parkeri colonization in the tick vector. Knockdown of the selenogenes triggered a compensatory response from other selenogenes, as observed by changes in gene expression, but oxidative stress levels and endoplasmic reticulum stress inside the ticks were also found to have heightened.
conclusionsThis study illustrates the potential of this new research model for augmenting our understanding of the pathogen interactions occurring within tick hosts and the important roles that symbionts and various tick factors play in regulating pathogen growth.
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