Evidence map›Paper›PMID 30103733›Full record

ArticleBMC cancer2018

Early increase in blood supply (EIBS) is associated with tumor risk in the Azoxymethane model of colon cancer.

Sarah Ruderman, Adam Eshein, Vesta Valuckaite, Urszula Dougherty, Anas Almoghrabi, Andrew Gomes, Ajaypal Singh, Baldeep Pabla, Hemant K Roy, John Hart and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 1 country.

Sarah RudermanDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
Adam EsheinDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.ORCID http://orcid.org/0000-0003-2582-0788
Vesta ValuckaiteCenter for Endoscopic Research and Therapeutics, University of Chicago Medicine, Chicago, IL, 60637, USA.
Urszula DoughertyCenter for Endoscopic Research and Therapeutics, University of Chicago Medicine, Chicago, IL, 60637, USA.
Anas AlmoghrabiCenter for Endoscopic Research and Therapeutics, University of Chicago Medicine, Chicago, IL, 60637, USA.
Andrew GomesDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
Ajaypal SinghDepartment of Gastroenterology, Rush University, Chicago, IL, 60612, USA.
Baldeep PablaCenter for Endoscopic Research and Therapeutics, University of Chicago Medicine, Chicago, IL, 60637, USA.
Hemant K RoyDepartment of Gastroenterology, Boston Medical Center, Boston, MA, 02118, USA.
John HartCenter for Endoscopic Research and Therapeutics, University of Chicago Medicine, Chicago, IL, 60637, USA.
Marc BissonnetteCenter for Endoscopic Research and Therapeutics, University of Chicago Medicine, Chicago, IL, 60637, USA.
Vani KondaCenter for Endoscopic Research and Therapeutics, University of Chicago Medicine, Chicago, IL, 60637, USA.
Vadim BackmanDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA. v-backman@northwestern.edu.
University of Chicago · USNorthwestern University · USBoston Medical Center · USRush University · US

Funding

ULTRASTRUCTURE AND CYTOMORPHOLOGY COREP30DK042086 · NIDDK · UNIVERSITY OF CHICAGO · PI ABADIE, VALERIE · 1990 to 2025
$29.8M
Biophotonics to Couple Pancreatic with Upper GI Screening via Ultrathin EndoscopyR01CA183101 · NCI · NORTHWESTERN UNIVERSITY · PI BACKMAN, VADIM, ROGERS, JEREMY D. · 2015 to 2019
$3.2M
Nanoscale/Molecular analysis of Fecal Colonocytes for Colorectal Cancer ScreeningR01CA165309 · NCI · NORTHWESTERN UNIVERSITY · PI BACKMAN, VADIM, ROY, HEMANT K. · 2012 to 2016
$2.8M
In-vivo characterization of pancreatic field carcinogenesis using spatially resolved reflectance measurements via a fiber optic probeF31EB022414 · NIBIB · NORTHWESTERN UNIVERSITY · PI ESHEIN, ADAM · 2016 to 2017
$76k
NCI NIH HHS R01 CA165309NCI NIH HHS R01CA165309NCI NIH HHS R01 CA183101NCI NIH HHS R01CA183101NIBIB NIH HHS F31 EB022414NIBIB NIH HHS F31EB022414
6 · The paper itself

Abstract

backgroundThe present study aimed to investigate the role of blood supply in early tumorigenesis in colorectal cancer. We leveraged the renin angiotensin system (RAS) to alter colonic blood supply and determine the effect on tumor initiation and progression.

methodsTo test the effect of blood supply on tumorigenesis, 53 male A/J mice were randomly assigned to one of three RAS modulation groups and one of two AOM treatments. The RAS modulation groups were I) water (RAS-unmodulated) as a control group, II) angiotensin-II and III) the angiotensin receptor blocker, Losartan. The mice in each group were then randomly split into either the saline control condition or the AOM-treated condition in which tumors were induced with a standard protocol of serial azoxymethane (AOM) injections. To monitor microvascular changes in the rectal mucosa during the study, we used confocal laser endomicroscopy (CLE) with FITC-Dextran for in-vivo imaging of vessels and polarization-gated spectroscopy (PGS) to quantify rectal hemoglobin concentration ([Hb]) and blood vessel radius (BVR).

resultsAt 12 weeks post-AOM injections and before tumor formation, CLE images revealed many traditional hallmarks of angiogenesis including vessel dilation, loss of co-planarity, irregularity, and vessel sprouting in the pericryptal capillaries of the rectal mucosa in AOM-Water tumor bearing mice. PGS measurements at the same time-point showed increased rectal [Hb] and decreased BVR. At later time points, CLE images showed pronounced angiogenic features including irregular networks throughout the colon. Notably, the AOM-Losartan mice had significantly lower tumor multiplicity and did not exhibit the same angiogenic features observed with CLE, or the increase in [Hb] or decrease in BVR measured with PGS. The AOM-AngII mice did not have any significant trends.

conclusionIn-vivo PGS measurements of rectal colonic blood supply as well as CLE imaging revealed angiogenic disruptions to the capillary network prior to tumor formation. Losartan demonstrated an effective way to mitigate the changes to blood supply during tumorigenesis and reduce tumor multiplicity. These effects can be used in future studies to understand the early vessel changes observed.

Indexed as

AnimalsAzoxymethaneBlood VesselsCarcinogenesisColonColonic NeoplasmsDextransDisease Models, AnimalFluorescein-5-isothiocyanateHemoglobinsHumansMiceMicroscopy, ConfocalRenin-Angiotensin SystemAzoxymethaneDextransFluorescein-5-isothiocyanatefluorescein isothiocyanate dextranHemoglobinsAngiogenesisColorectal cancerEarly increase in blood supplyField effect of carcinogenesisRenin angiotensin system

Identifiers

PMID30103733
PMCPMC6090821
OpenAlexW2887543367

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.